DNA Damage and Chromosomal Instability, Volume 182 in the Methods in Cell Biology series, presents interesting chapters on a variety of timely topics, including Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivation, Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agents, Genetic reporters to detect and quantify homologous recombination in yeast, Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variants, Enrichment of DNA replication intermediates by EdU pull down, Nascent DNA sequencing and its diverse applications in genome integrity research, and much more. Additional chapters cover Enrichment of DNA replication intermediates by EdU pull down, DNA curtains for studying phase separation mechanisms of DNA-organizing proteins, Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule Level, Cloning and Expansion of Repetitive DNA Sequences, Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopy Arne Lindqvist Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Cell Biology seriesUpdated release includes the latest information on the DNA Damage and Chromosomal Instability INDICE: 1. Induction of chromosome-specific micronuclei and chromothripsis by centromere inactivationPeter Ly2. Generation of aneuploid cells and assessment of their ability to survive in presence of chemotherapeutic agentsStefano Santaguida3. Genetic reporters to detect and quantify homologous recombination in yeastLoraine Symington4. Mapping histone variant genomic distribution: exploiting SNAP- Tag labelling to follow the dynamics of incorporation of H3 variantsGeneviève Almouzni and Jean-Pierre Quivy5. Nascent DNA sequencing and its diverse applications in genome integrity researchAndre Nussenzweig and Jacob Paiano6. Enrichment of DNA replication intermediates by EdU pull downYlli Doksani7. DNA curtains for studying phase separation mechanisms of DNA-organizing proteinsJohannes Stigler8. Visualizing the Dynamics of DNA Replication and Repair at the Single-Molecule Molecule LevelGheorghe Chistol9. Cloning and Expansion of Repetitive DNA SequencesGideon Coster and Sophie Lyn Williams10. Using an ImageJ-based script to detect replication stress and associated cell cycle exit from G2 phase by fluorescence microscopyArne Lindqvist11. An automated image analysis pipeline to quantify the coordination and overlap of transcription and replication activity in mammalian genomesStephan Hamperl12. Monitoring Chk1 kinase activity dynamics in live single cell imaging assaysOlivier Gavet and Vicente Lebrec13. Visualising DNA damage and repair using single molecule super resolution microscopyDonna Whelan14. Quantification of nuclear deformations and DNA damage foci dynamics by live-cell imagingFrancisca Lottersberger15. Native FISH: A low- and high- throughput assay to analyze the Alternative Lengthening of Telomere (ALT) pathwayEros Lazzerini-Denchi and Benura Azeroglu16. Assessment of DNA FibresChris Staples17. A high-content flow cytometry and dual CRISPR-Cas9 based platform to quantify genetic interactions Priyanka Verma 18. Monitoring APOBEC3A protein levels in human cancer cellsJohn Maciejowski
- ISBN: 978-0-443-18898-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 318
- Fecha Publicación: 12/02/2024
- Nº Volúmenes: 1
- Idioma: Inglés