Since the publication of the first edition, the techniques and experimental approaches employed in the study of adipose tissue have undergone radical expansion and revision. In Adipose Tissue Protocols: Second Edition, several updated chapters are combined with many new contributions from internationally recognized experts covering both conventional and state-of-the-art methodologies aswell as whole animal, cellular and molecular approaches which have emerged inor been extended to adipose tissue research over the past few years. As a volume in the highly sucessful Methods in Molecular Biology™ series, each chapterincludes an introduction to its subject matter, a list of necessary materials, step-by-step, readily reproducible laboratory protocols, and a Notes section, which provides invaluable tips and tricks to safeguard against potential mishaps and pitfalls. An indispensable, all-in-one manual for those interested inconducting any aspect of adipose tissue research Collects all essential techniques with readily reproducible, step-by-step protocols to ensure success Provides the most up-to-date methods in clear detail INDICE: From the contents Overview of Adipose Tissue and Its Role in Obesity and Metabolic Disorders.- Choosing an Adipose Tissue Depot for Sampling: Factors in Selection and Depot Specificity.- Application of Imaging and Other Non-invasive Techniques in Determining Adipose Tissue Mass.- Generation of Adipose Tissue-Specific Transgenic Mouse Models.- Angiogenesis in Adipose Tissue.- Adipose Organ Nerves Revealed by Immunohistochemistry.- Human Adipose Tissue Blood Flow and Micromanipulation of Human Subcutaneous Blood Flow.- Studies of Thermogenesis and Mitochondrial Function in Adipose Tissues.- Application of Lipidomics and Metabolomics to the Study of Adipose Tissue.- Applications of Proteomics to the Study of Adipose Tissue.- Application of DNA Microarray to theStudy of Human Adipose Tissue/Cells.- Differentiation of Adipose Stem Cells.-Methods That Resolve Different Contributions of Clonal Expansion to Adipogenesis in 3T3-L1 and C3H10T1/2 Cells.- Explant Cultures of White Adipose Tissue.
- ISBN: 978-1-58829-916-1
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 400
- Fecha Publicación: 01/07/2008
- Nº Volúmenes: 1
- Idioma: Inglés