This new volume of Methods in Enzymology continues the legacy of this premier serial by containing quality chapters authored by leaders in the field. The first of 2 volumes covering nucleosomes, histones and chromatin, it has chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins. Continues the legacy of this premier serial by containing quality chapters authored by leaders in the field The first of 2 volumes covering nucleosomes, histones and chromatin Chapters on methods applied to the study of protein arginine methylation, high-resolution identification of intra- and interchromosomal DNA interactions by 4C technology, and peptide arrays to interrogate the binding specificity of chromatin-binding proteins INDICE: 1. Methods applied to the study of Protein Arginine Methylation 2. TBC 3. Genome-wide mapping of nucleosomes in budding yeast 4. High resolution identification of intra- and interchromosomal DNA interactions by 4C technology 5. TBC 6. High throughput assays to identify small-molecule inhibitors of chromatin 'reader' proteins 7. Peptide microarrays to interrogate the â?~histone codeâ?T 8. Preparation of Drosophila cells from different stages of the cell cycle for ChIP using centrifugal elutriation and FACS 9. Analysis of Single and Combinatorial Histone PTM Patterns by High Resolution Mass Spectrometry 10. Measuring nucleosome turnover dynamics genome-wide 11. Comparative mRNA expression-profiling of chromatin factors 12. High-Throughput MAPit Single-Molecule Footprinting 13. Genome-wide in vitro reconstitution 14. Quantitating interactions of nuclear factors with chromatin 15. Biochemical assay for histone H2AZ (Htz1) replacement by the yeast SWR1 chromatin remodeling complex 16. TBC 17. TBC
- ISBN: 978-0-12-391940-3
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 390
- Fecha Publicación: 11/10/2012
- Nº Volúmenes: 1
- Idioma: Inglés