In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular Biology™ series, each chapter provides a briefintroduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Includes CD with full colorreproductions of many figures within the book Contains simple and complete protocols for culturing osteoblasts and osteoclasts Explains how to utilize various rodent models to study and treat osteoporosis Provides protocols for increasing or decreasing gene expression in bone cells Examines molecular, genetic and imaging techniques to study bone INDICE: From the contents Isolation and Culture of Rodent Osteoprogenitor Cells.- Osteoclast Culture and Resorption Assays.- Assessing New Bone Formation in Neonatal Calvarial Organ Cultures.- Detection of Apoptosis of Bone Cells In Vitro.- In Vivo Parathyroid Hormone Treatments and mRNA Isolation and Analysis.- Assessment of Bone Formation Capacity Using In Vivo Transplantation Assays: Procedure and Tissue Analysis.- Ossicle and Vossicle Implant Model Systems.- Utility of the Ovariectomized (OVX) Rat as a Model for Human Osteoporosis In Drug Discovery.- Orchidectomy (ORX) Models of Osteoporosis.- Gene Delivery by Adenoviruses.- Lentiviral Delivery of shRNA Constructs into Osteoblasts.- Gene Delivery by Retroviruses.- Chromatin Immunoprecipitation (ChIP) Assays: Application of ChIP-on-chip for Defining Dynamic Transcriptional Mechanisms in Bone Cells.- Identification of Transcription Factor Target Genes by ChIP Display(CD).
- ISBN: 978-1-58829-828-7
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 425
- Fecha Publicación: 01/06/2008
- Nº Volúmenes: 1
- Idioma: Inglés