Recent advances in the biosciences have led to a range of powerful new technologies, particularly nucleic acid, protein and cell-based methodologies. The most recent insights have come to affect how scientists investigate and define cellular processes at the molecular level. Molecular Biomethods Handbook, 2nd Edition expands upon the techniques included in the first edition, providing theory, outlines of practical procedures, and applications for a range of techniques. Part A of the book describes nucleic acid methods, such as gene expression profiling, microarray analysis and quantitative PCR. In Part B, protein and cell-based methods are outlined, in subjects ranging from protein engineering to high throughput screening. Written by a well-established panel of research scientists, Molecular Biomethods Handbook, 2nd Edition provides an up to date collection of methods used regularly in the authors’ own research programs. Comprehensive collection of wide-ranging molecular and biochemical techniques frequently used in medical and clinical research laboratories around the worldCovers a wide array of techniques and protocols used for diagnostics Includesstep-by-step reproducible laboratory protocols written by experts in the field and includes a notes section that outlines tricks of the trade INDICE: From the contents Part A: Nucleic Acid Biomethods: The manipulation of nucleic acids: basic tools and techniques.- Restriction Enzymes.- Principles and Medical Applications of the Polymerase Chain Reaction.- Probe Design, Production, and Applications.- Southern Blotting as a Diagnostic Method.- Introduction to Capillary Electrophoresis of DNA.- Denaturing High-Performance Liquid Chromatography (DHPLC) for nucleic acid analysis.- Denaturing Gradient GelElectrophoresis (DGGE).- Single Strand Conformation Polymorphism (SSCP) Analysis.- Randomly amplified polymorphic DNA (RAPD); a useful tool for genomic characterization of different organisms.- Quantification of mRNA using Real Time RT-PCR – the SYBR solution.- Quantitative Analysis of DNA Sequences by PCR andSolid Phase Sequencing.- MAPH (Multiplex Amplifiable Probe Hybridization).- Gene Expression Profiling.- Comparative Genomic Hybridization in Clinical and Medical Research.- Subtractive Hybridization.- Fluorescence in situ Hybridization.
- ISBN: 978-1-60327-370-1
- Editorial: Humana Press
- Encuadernacion: Cartoné
- Páginas: 1124
- Fecha Publicación: 01/06/2008
- Nº Volúmenes: 1
- Idioma: Inglés