Retaining the proven didactic concept of the successful Chemical Biology – Learning Through Case Studies, this sequel features 20 new case studies, reflecting the rapid growth in this interdisciplinary topic over the past few years. Edited by one of the world?s leading researchers in the field, this textbook introduces students and researchers to the modern approaches in chemical biology, as well as important results, and the techniques and methods applied. Each chapter presents a different biological problem taken from everyday lab work, elucidated by an international team of renowned scientists. With its broad coverage, this is a valuable source of information for students, graduate students, and researchers working on the borderline between chemistry, biology, and biochemistry. INDICE: Real–Time and Continuous Sensors of Protein Kinase Activity Utilizing Chelation–Enhanced Fluorescence FLiK and FLiP: Direct Binding Assays for the Identification of Stabilizers of Inactive Kinase and Phosphatase Conformations Strategies for Designing Specific Protein Tyrosine Phosphatase Inhibitors and their Intracellular Activation Design and Application of Chemical Probes for Protein Serine/Threonine Phosphatase Activation Autophagy: Assays and Small Molecule Modulators Elucidation of Protein Function by Chemical Modification Inhibition of Oncogenic K–Ras Signaling by Targeting K–Ras–PDEDelta Interaction Development of Acyl Protein Thioesterase 1 (APT1) Inhibitor Palmostatin B that Revert Unregulated H/N–Ras Signaling Functional Analysis of Host–Pathogen Posttranslational Modification Crosstalk of Rab Proteins Chemical Biology Approach to Suppression of Statin–Induced Muscle Toxicity A Target Identification System Based on MorphoBase, ChemProteoBase, and Photo–Crosslinking Beads Activity–Based Proteasome Profiling in Medicinal Chemistry and Chemical Biology Rational Design of Activity–Based Retaining Beta–Exoglucosidase Probes Modulation of ClpP Protease Activity: From Antibiotics to Anti–Virulence Affinity–Based Isolation of Molecular Targets of Clinically used Drugs Identification of the Targets of Natural Product–Inspired Mitotic Inhibitors Finding a Needle in a Haystack. Identification of Tankyrase a Novel Therapeutic Target of the Wnt Pathway using Chemical Genetics The Identification of the Molecular Reporter of the Plant Hormone Abscisic Acid Chemical Biology in Plants: Finding New Connections between Pathways using the Small Molecule Sortin 1 Selective Targeting of Protein Interactions Mediated by BET Bromodomains The Impact of Distant Polypharmacology in the Chemical Biology of PARPs Splicing Inhibitors: From Small Molecule to RNA Metabolism Photochemical Control of Gene Function in Zebrafish Embryos with Light–Activated Morpholinos Life Cell Imaging of mRNA using PNA FIT–Probes Targeting the Transcriptional Hub Beta–Catenin using Stapled Peptides Diversity Oriented Synthesis: Developing New Chemical Tools to Probe and Modulate Biological Systems Scaffold Diversity Synthesis with Branching Cascades Strategy
- ISBN: 978-3-527-33611-1
- Editorial: Wiley VCH
- Encuadernacion: Rústica
- Páginas: 464
- Fecha Publicación: 13/08/2014
- Nº Volúmenes: 1
- Idioma: Inglés