The field of clinical proteomics has rapidly evolved during the past few years and is continuously growing as new methodologies and technologies emerge. InClinical Proteomics, a select group of leading researchers has contributed their state-of-the-art methodologies on protein profiling and identification of disease biomarkers in tissues, microdissected cells and body fluids. Experimental approaches involving the application of two-dimensional electrophoresis, multi-dimensional liquid chromatography, SELDI/MALDI mass spectrometry andprotein arrays, as well as the bioinformatic and statistical tools pertinent to the analysis of proteomics data, are thoroughly detailed in these readily reproducible methods and protocols, explained through the step-by-step instructional format of the highly successful Methods in Molecular Biology™ series. Step-by-step instructions that ensure successful results. Integrates biochemistry, pathology, analytical technology, bioinformatics, and proteome informatics. INDICE: From the contents Overview and Introduction to Clinical Proteomics.- Specimen collection and handling: Standardization of blood sample collection.- Tissue Sample Collection for Proteomics Analysis.- Protein profiling of Human Plasma Samples by Two-Dimensional Electrophoresis.- Analysis of Laser Capture Microdissected Cells by 2-Dimensional Gel Electrophoresis.- Optimizing theDifference Gel Electrophoresis (DIGE) technology.- MALDI/SELDI protein profiling of serum for the identification of cancer biomarkers.- Urine sample preparation and protein profiling by two-dimensional electrophoresis and matrix assisted laser desorption ionization time-of-flight mass spectrometry.- Combining Laser Capture Microdissection and proteomics techniques.- Comparison of Protein Expression by Isotope-Coded Affinity Tag Labelling.- Analysis of microdissected cells by two-dimensional LC-MS approaches.- Label-free LC-MS Method for the Identification of Biomarkers.
- ISBN: 978-1-58829-837-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 480
- Fecha Publicación: 01/04/2008
- Nº Volúmenes: 1
- Idioma: Inglés