Wnt Signaling: Methods and Protocols examines both biochemical assays and vertebrate and invertebrate model systems to provide a point of reference to current molecular protocols and the diverse model systems employed to study this important signaling pathway. In Volume 2, Pathway Models, the diverse vertebrate and invertebrate models that have shaped the Wnt signaling field are described, presenting an overview of the unique properties of each organism, like asymmetric cell division in C. elegans and epithelial morphogenesis in Dictyostelium, with respect to studying Wnt/FZD function. As a volume in the highly successful Methods in Molecular Biology™ series, chapters contain readily reproducible laboratory protocols, complete with lists of necessary equipment and reagents and the Notes section, which reveals helpful troubleshooting tips. Comprehensive and cutting-edge, Wnt Signaling: Methods and Protocols collects the expertise and knowledge of many leaders in the field. Records our progress to date in understanding the intricacies of Wnt signalling Includes assays for and models of both canonical and non-canonical Wnt signalling pathways Contributing authors are either current or past members of the main laboratories that have shaped the Wnt field Includes detailed methods as well as overviews, providing easy to follow protocols as well as a background/current status of the field INDICE: From the contents Evolution of the Wnt pathway.- Dictyostelium development: a prototypic Wnt pathway?.- Monitoring patterns of gene expression in Dictyostelium by b -galactosidase staining.- Use of the Dictyostelium stalk cell assay to monitor GSK-3 regulation.- Wnt signaling in Cnidarians.- Detecting expression patterns of Wnt pathway components in Nematostella vectensis embryos.- Detection of expression patterns in Hydra pattern formation.- Analysis of Wnt signaling pathways during C. elegans postembryonic development.- Wnt signaling during C. Elegans embryonic development.- Function of the Wingless signaling pathway in Drosophila.- Visualisation of PCP defects in the eye and wing of Drosophila melanogaster.- Wingless signaling in Drosophila eye development.- High-throughput RNAi screen in Drosophila.- Wnt signaling in early sea urchin development.- Detecting expression patterns of Wnt pathway components in sea urchin embryos using whole mount in situ hybridization.
- ISBN: 978-1-58829-912-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 465
- Fecha Publicación: 01/09/2008
- Nº Volúmenes: 1
- Idioma: Inglés