Instrumental analysis of intrinsically disorderedproteins: assessing structure and conformation

The number of proteins and protein domains, that have been shown to be intrinsically disordered, is rapidly amplifying. Although the significant achievements in methods suitable for characterization of these intriguing members of theprotein realm have been presented in several reviews, they have never being the subject of a book. This book is to fill this gap and to summarize recent achievements in the methods for structural characterization of intrinsically disordered proteins. The book will address structural biology and protein degradation, and then address a myriad of spectroscopic techniques that can be used to elucidate protein structure: NMR and EPR spectroscopies, FTIR, circular dichroism, fluorescence spectroscopy, vibrational methods, and single-molecule analysis. Methods that can help to assess protein size and shape will follow, as well as a section discussing conformational stability issues that relate specifically to these more flexible proteins.Vladimir N. Uversky is a Senior Research Professor at the Indiana University School of Medicine. He obtained his academic degrees from Moscow Institute of Physics and Technology (Ph.D.) and from the Institute of Experimental and Theoretical Biophysics, Russian Academy of Sciences. He spent most of his career working for Institute of Protein Research and Institute for Biological Instrumentation (Russia). Since 1998 he worked for UCSC before taking his present appointment at IU in 2004. Professor Uversky has authored over 250 scientific publications and edited several books and book series on protein structure, function, folding and misfolding. He is also an editor of several scientific journals. Sonia Longhi is a Director of Research at the CNRS (Center for the NationalScientific Research). She obtained her academic degree (PhD) from the Universita' degli Studi of Milan (Italy) in 1993. She then went to the laboratory Architecture and Function of Biological Macromolecules (AFMB,CNRS and Universities Aix-Marseille I and II) where she did a post-doctoral stay working on protein crystallography. In 1999 she got a permanent position as a senior scientist within the CNRS. Since 2006 she is the head of a group titled ‘Structural Disorder within Viruses’ within the AFMB laboratory. In 2007 she got a position asa permanent director of research within the CNRS. She has authored more than 50 scientific publications and edited a book on measles virus nucleoprotein.

• ISBN: 978-0-470-34341-8
• Editorial: John Wiley & Sons
• Encuadernacion: Cartoné
• Páginas: 752
• Fecha Publicación: 07/05/2010
• Nº Volúmenes: 1
• Idioma: Inglés