Agrobacterium is a plant pathogen which causes the ‘crown-gall’ disease, a neoplastic growth that results from the transfer of a well-defined DNA segment (‘transferred DNA’, or ‘T-DNA’) from the bacterial Ti (tumor-inducing) plasmid to the host cell, its integration into the host genome, and the expression of oncogenes contained on the T-DNA. The molecular machinery, needed for T-DNA generation and transport into the host cell and encoded by a series of chromosomal (chv) and Ti-plasmid virulence (vir) genes, has been the subject of numerous studies over the past several decades. Today, Agrobacterium is the tool of choice for plant genetic engineering with an ever expanding host range that includes many commercially important crops, flowers, and tree species. Furthermore, its recent application for the genetic transformation of non-plant species,from yeast to cultivated mushrooms and even to human cells, promises this bacterium a unique place in the future of biotechnological applications. The onlycomplete overview of all aspects of agrobacterium in the plant sciences Dealswith various aspects of the biology of the diseases caused by agrobacterium Covers the basics of the bacteria itself, the mapping of its genome, through toits use in plant transformation and biotechnology Delves into the questions of ethics and law in the biotechnological applications of Agrobacterium INDICE: From the contents Agrobacterium: a disease-causing bacterium.- A brief history of research on Agrobacterium tumefaciens: 1900-1980.- Agrobacterium and plant biotechnology.- The Agrobacterium tumefaciens C58 genome.- Agrobacterium–taxonomy of plant-pathogenic Rhizobium species.- The initial steps in Agrobacterium tumefaciens pathogenesis: chemical biology of host recognition.-Agrobacterium-host attachment and biofilm formation.- Production of a mobile T-DNA by Agrobacterium tumefaciens.- Translocation of oncogenic T-DNA and effector proteins to plant cells.- Intracellular transport of Agrobacterium T-DNA.- Mechanisms of T-DNA integration.- Agrobacterium tumefaciens-mediated transformation: patterns of T-DNA integration into the host genome.- Function of hostproteins in the Agrobacterium-mediated plant transformation process.- The oncogenes of Agrobacterium tumefaciens and Agrobacterium rhizogenes.
- ISBN: 978-0-387-72289-4
- Editorial: Springer
- Encuadernacion: Cartoné
- Páginas: 750
- Fecha Publicación: 01/04/2008
- Nº Volúmenes: 1
- Idioma: Inglés