Planar cell polarity (PCP), or the alignment of a collection of cells within a cell sheet, has proven, over time, to be vital in not only normal development but also in disease states. In Planar Cell Polarity: Methods and Protocols,expert researchers in the field present a number of detailed and well-designed protocols used successfully in their labs around the world. As a volume in the highly successful Methods in Molecular Biology? series, the chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, Planar Cell Polarity: Methods and Protocols serves as a valuable reference for both novices and experts in this dynamic and versatile area of study. INDICE: 1. Live Imaging of Drosophila Embryos: Quantifying Protein Numbersand Dynamics at Subcellular Locations. Daryl J.V. David, Melanie A. McGill, R.F. Andrew McKinley, and Tony J.C. Harris. 2. Analyzing Frizzled Signaling Using Fixed and Live Imaging of the Asymmetric Cell Division of the Drosophila Sensory Organ Precursor Cell. Bertrand Jauffred and Yohanns Bellaiche. 3. Protein-Protein Interaction Techniques: Dissect PCP Signaling in Xenopus. Yingqun Wang. 4. Cuticle Refraction Microscopy (CRM): A Rapid and Simple Method for Imaging Drosophila Wing Topography, an Alternative Readout of Wing Planar Cell Polarity. David Neff, Justin Hogan, and Simon Collier. 5. Analysis of Cell Shape and Polarity during Zebrafish Gastrulation. Douglas C. Weiser and David Kimelman. 6. Analyzing Planar Cell Polarity during Zebrafish Gastrulation. Jason R. Jessen. 7. Wnt/Planar Cell Polarity Signaling in the Regulation of Convergent Extension Movements during Xenopus Gastrulation. Gun-Hwa Kim, Edmond ChangkyunPark, and Jin-Kwan Han. 8. Using 32-Cell Stage Xenopus Embryos to Probe PCP Signaling. Hyun-Shik Lee, Sergei Y. Sokol, Sally A. Moody, and Ira O. Daar. 9. Gene Loss-of-Function and Live Imaging in Chick Embryos. Anne C. Rios, Christophe Marcelle, and Olivier Serralbo. 10. Activation and Function of Small GTPases Rho, Rac, and Cdc42 during Gastrulation. Courtney Mezzacappa, Yuko Komiya, and Raymond Habas. 11. Convergent Extension Analysis in Mouse Whole Embryo Culture. Sophie E. Pryor, Valentina Massa, Dawn Savery, Nicholas D.E. Greene, andAndrew J. Copp. 12. Analysis of PCP Defects in Mammalian Eye Lens. Yuki Sugiyama and John W. McAvoy. 13. Examining Planar Cell Polarity (PCP) in the Mammalian Cochlea. Helen May-Simera and Matthew W. Kelley. 14. Role of Prickle1 and Prickle2 in Neurite Outgrowth in Murine Neuroblastoma Cells. Lisa Fujimura andMasahiko Hatano. 15. The Planar Cell Polarity Pathway and Parietal Endoderm Cell Migration. Kristi LaMonica and Laura Grabel. 16. Analysis of Wnt/Planar Cell Polarity Pathway in Cultured Cells. Mitsuharu Endo, Michiru Nishita, and Yasuhiro Minami. 17. Regulation of Focal Adhesion Dynamics by Wnt5a Signaling. Shinji Matsumoto and Akira Kikuchi. 18. The Embryonic Mouse Gut Tube as a Modelfor Analysis of Epithelial Polarity. Makoto Matsuyama and Akihiko Shimono. 19. Assessing PCP in the Cochlea of Mammalian Ciliopathy Models. Daniel J. Jagger and Andrew Forge. 20. Morphometric Analysis of Centrosome Position in Tissues. Hester Happé, Emile de Heer, and Dorien J.M. Peters.
- ISBN: 978-1-61779-509-1
- Editorial: Humana
- Encuadernacion: Rústica
- Fecha Publicación: 07/01/2012
- Nº Volúmenes: 1
- Idioma: Inglés