Over the past few years, DNA methylation technologies and our knowledge of DNA methylation patterns have been advancing at a breathtaking pace. Due to thisfact, DNA Methylation: Methods and Protocols, Second Edition completely revises, updates, and expands upon the popular first edition with the most current novel techniques, easier to use and more refined by the tested experience of leading experts. This revision reflects contemporary study of the subject: the analysis of gene-specific DNA methylation patterns has been complemented by genome-wide approaches, and epigenomics takes a central place. Written in the highly successful Methods in Molecular Biology™ series format, the chapters in this volume present brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes which highlight tips on troubleshooting and avoiding known pitfalls Provides an easily accessible reference volume for DNA methylation technologies as written by leading experts in the field Focuses on validated and breakthrough innovations for all major questions of development and disease-related DNA methylation research Details methods usable for every laboratory in the world and ensures the rapid implementation of DNA methylation analysis in the laboratory INDICE: From the contents DNA Methylation: An Introduction to the Biology and the Disease-Associated Changes of a Promising Biomarker.- Quantification of Global DNA Methylation by Capillary Electrophoresis and Mass Spectrometry.- Methyl Group Acceptance Assay for the Determination of Global DNA Methylation Levels.- Immunodetection Array.- Methylated DNA Immunoprecipitation (MeDIP).- The MIRA Method for DNA Methylation Analysis.- The HELP Assay.- Differential Methylation Hybridization: Profiling DNA Methylation with a High Density CpG Island Microarray.- Analysis of DNA Methylation by Amplification of InterMethylated Sites (AIMS).- Methylation-Sensitive Representational Difference Analysis (MS-RDA).- Restriction Landmark Genomic Scanning: Analysis of CpG Islands in Genomes by 2D Gel Electrophoresis.- GoldenGate® Assay for DNA Methylation Profiling.- 5’-Azacytidine Expression Arrays.- DNA Methylation Analysis by Bisulfite Conversion, Cloning, and Sequencing of Individual Clones.
- ISBN: 978-1-934115-61-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 330
- Fecha Publicación: 01/12/2008
- Nº Volúmenes: 1
- Idioma: Inglés