Functional proteomics: methods and protocols

As the emerging field of proteomics continues to expand at an extremely rapidrate, the relative quantification of proteins, targeted by their function, becomes its greatest challenge. Complex analytical strategies have been designedthat allow comparative analysis of large proteomes, as well as in depth detection of the core proteome or the interaction network of a given protein of interest. In Functional Proteomics: Methods and Protocols, expert researchers describe the latest protocols being developed to address the problems encounteredin high-throughput proteomics projects, with emphasis on the factors governing the technical choices for given applications. Unique combination of reproducible laboratory protocols and state-of-the-art bioinformatics methods dedicated to functional proteomics A comprehensive methodological compendium, coveringsample preparation, protein separation, mass-spectrometry as well as data mining, documentation and integration Offers methods, workflows and strategies toovercome current pitfalls and shortcomings in proteome analysis INDICE: From the contents A Brief Summary of the Different Types of Mass Spectrometers Used in Proteomics.- Experimental Setups and Considerations to Study Microbial Interactions.- Plant Proteomics.- Methods for Human CD8+ T Lymphocyte Proteome Analysis.- Label-Free Proteomics of Serum.- Flow Cytometric Analysis of Cell Membrane Microparticles.- Exosomes.- Toward a Full Characterization of the Human 20S Proteasome Subunits and Their Isoforms by a Combination of Proteomic Approaches.- Free-Flow Electrophoresis of the Human Urinary Proteome.- Versatile Screening for Binary Protein–Protein Interactions by Yeast Two-Hybrid Mating.- Native Fractionation: Isolation of Native Membrane-Bound Protein Complexes from Porcine Rod Outer Segments Using Isopycnic Density Gradient Centrifugation.- Mapping of Signaling Pathways by Functional Interaction Proteomics.- Selection of Recombinant Antibodies by Eukaryotic Ribosome Display.- Production of Protein Arrays by Cell-Free Systems.

• ISBN: 978-1-58829-971-0
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 590
• Fecha Publicación: 01/08/2008
• Nº Volúmenes: 1
• Idioma: Inglés