The Immunological Synapse - Part C, Volume 190 in the Methods in Cell Biology series provides state-of-the-art and detailed methods for the study of the T cell, natural killer (NK) cell and B cell immunological synapses. Topics covered in this third volume include tau-STED microscopy for 3D-quantitative colocalization of lytic granule markers, image processing approaches for 3D-analysis of markers and for microtubule remodeling quantification at the immunological synapse, flow cytometry to analyze NK cell-target cell conjugation, imaging flow cytometry to quantify a range of different subcellular processes at the immunological synapse, imaging techniques aimed at quantitatively analyzing B cell immunological synapse formation, quantification of force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensors, measuring interaction forces between T lymphocytes and their target cells using live microscopy and laminar shear flow chambers, measuring interaction kinetics between T cells and their target tumor cells with optical tweezers, and gauging antigen recognition by human primary T-cells featuring orthotopically exchanged TCRs of choice. Additionally, this volume includes methods to isolate and characterize primary NK cells and enrich the KIR2DL1+ population, and to expand ?d T cells and evaluate their cytotoxicity. Covers various methods related to the study of the immunological synapse Includes detailed, point-by-point, methods as well as various important notesProvides the authority and expertise from an international board of leading scientists INDICE: PrefaceClément Thomas and Lorenzo Galluzzi1. Analysis of immune synapse by STED imaging and 3D quantitative colocalization of lytic granule markersClaudio Giraudo2. Imaging the immune synapse: three-dimensional analysis of the immune synapseManuel Izquierdo3. Image processing approaches for microtubule remodeling quantification at the immunological synapseAndres Alcover, Marie Juzans, Celine Cuche and Vincenzo Di-Bartolo4. Isolation and characterization of primary NK cells and their KIR2DL1+ population enrichmentMira Barda-Saad5. Chapter title to be confirmedJohannes B. Huppa and Janett Göhring6. Flow Cytometry Conjugate Formation Assay between Natural Killer (NK) Cells and Their Target CellsJacques Zimmer7. Quantifying force-mediated antigen extraction in the B cell immune synapse using DNA-based tension sensorsKatelyn Spillane, Anna Bajur and Hannah McArthur8. Chapter title to be confirmedRose Thierry9. Quantitative analysis of the B Cell immune synapse formation using imaging techniquesIsabel Yuseff10. chapter title yet to confirmedRose Thierry11. Expansion of ?d T cells and their use in in vitro cytotoxicity assaysMaria Alejandra Parigiani12. A Comprehensive Guide to Studying the Immunological Synapse Using Imaging Flow CytometryCéline Hoffmann, Clément Thomas, Liza Filali, Andrea Michela Biolato and Max Krecke
- ISBN: 978-0-443-21868-2
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 312
- Fecha Publicación: 15/01/2025
- Nº Volúmenes: 1
- Idioma: Inglés