Through a variety of mechanisms, cancer cells provide the biomarker material that can lead to their own detection, which then grants clinicians the opportunity for non-invasive body fluid and tissue analysis able to indicate the presence of tumors or the level of tumor burden. In Tumor Biomarker Discovery: Methods and Protocols, experts in the field present detailed chapters focused on the use and development of panels of biomarker analytes while covering subjects such as nucleic acids and protein-based technologies, metabolic profiling byanalytic means or spectroscopy, as well as study designs for biomarker discovery and validation. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of the necessary materials or reagents, step-by-step laboratory protocols, andnotes on troubleshooting and avoiding known pitfalls. Provides a detailed overview of the range of biomarker technologies as written by leading researchersin the field Useful both for researchers interested in new tumor biomarkers and physicians seeking familiarity with new technologies in the clinical setting Covers a wide range of biomarker assay formats and systems Comprehensive guide to pitfalls of biomarker discovery and validation technologies INDICE: From the contents Identification of Tumor-Associated Antigens (TAAs) as Diagnostic and Predictive Biomarkers in Cancer.- Autoantibodies against Cancer Antigens.- Discovery of Antibody Biomarkers Using Protein Microarrays of Tumor Antigens Cloned in High-Throughput.- Analysis of Glycans on Serum Proteins Using Antibody Microarrays.- Glycoproteomic Analysis by Two Dimensional Electrophoresis.- All-Liquid Separations, Protein Microarrays and Mass Spectrometry to Interrogate Serum Proteomes: An Application to Serum Glycoproteomics.-Reverse Phase Protein Microarrays for Theranostics and Patient Tailored Therapy.- Serum Proteomics Using Mass Spectrometry.- Hormones as Biomarkers: Practical Guide to Utilizing Luminex Technolgies for Biomarker Research.- High-Throughput Analysis of Serum Antigens Using Sandwich ELISAs on Microarrays.- TissueMicroarrays as a Tool in the Discovery and Validation of Tumor Markers.- Quantitative, Fluorescence-Based In-Situ Assessment of Protein Expression.
- ISBN: 978-1-60327-810-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 300
- Fecha Publicación: 01/03/2009
- Nº Volúmenes: 1
- Idioma: Inglés