Since the publication of the first edition, the number of unique heritable mtDNA mutations recognized as being associated with bioenergetic dysfunction, cell death and disease has grown. Likewise, our understanding of the basic biology of somatic mtDNA mutations continues to improve. In Mitochondrial DNA: Methods and Protocols, Second Edition, specialists from eight countries share their expertise, providing detailed protocols for studying many aspects of mtDNA. The volume is divided into three sections, which cover the transduction of information from mtDNA to functionally active respiratory complexes, mitochondrial reactive oxygen species (ROS) production as well as mtDNA damage and its repair, and the identification and quantification of heteroplasmic mtDNA mutations. Covers a wide range of methods for investigating how mitochondrial geneticsaffect cellular physiology and human health Details unique experimental approaches for studying mtDNA damage and mutation Experimental resource for researchers and physicians concerned with mtDNA disorders Provides an in depth description of tools and approaches used by leading researchers INDICE: From the contents Biochemical Isolation of mtDNA Nucleoids from Animal Cells.- Analysis of Mitochondrial DNA by Two Dimensional Agarose Gel Electrophoresis.- Comparative Purification Strategies for Drosophilia and Human Mitochondrial DNA Replication Proteins: DNA Polymerase y and Mitochondrial Single-Stranded DNA-Binding Protein.- Functional Analysis of Mutant Mitochondrial DNA Polymerase Proteins Involved in Human Disease.- Preparation of Human Mitochondrial Single-Stranded DNA Binding Protein.- Methods for Assessing Binding ofMitochondrial Transcription Factor A (TFAM) to DNA.- Inducible Expression in Human Cells, Purification and in vitro Assays for the Mitochondrial DNA Helicase Twinkle.- Purification Strategy for Recombinant Forms of the Human Mitochondrial DNA Helicase.- Methods for Studying Mitochondrial Transcription Termination with Isolated Components.
- ISBN: 978-1-934115-60-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 360
- Fecha Publicación: 01/06/2009
- Nº Volúmenes: 1
- Idioma: Inglés