Since the first edition, published in 2001, genomics research has taken greatstrides. In Genomics Protocols, 2nd edition, a team of expert researchers share the most current information in a field that has recently switched emphasisfrom gene identification to functional genomics and the characterization of genes and gene products. This volume approaches its complex subject with a broad perspective to supply its reader with a vital overview of genomics and its derivative fields, with a focus on pivotal issues such as data analysis. Following the highly successful Methods in Molecular Biology™ series format, the chapters provide easy-to-follow laboratory protocols, lists of the necessary materials, and the excellent Notes section, which offers tips on troubleshooting and avoiding known pitfalls. Expansive and current, Genomics Protocols, 2nd edition is the perfect update for researchers working in this integral, growing field. Unparalleled diversity of technologies covered in a single volume INDICE: From the contents Whole Genome Amplification with Phi29 DNA Polymerase to enable Genetic or Genomic Analysis of Samples of Low DNA Yield.- Scanning for DNA variants by denaturant capillary electrophoresis.- Identification of SNPs, or Mutations in Sequence Chromatograms.- Beadarray based Genotyping.-Microsatellite-Based Candidate Gene Linkage Analysis Studies.- Full Complexity Genomic Hybridization on 60mer Oligonucleotide Microarrays for Array Comparative Genomic Hybridization (aCGH).- Detection of Copy Number Changes at Multiple Loci in DNA Prepared from Formalin Fixed, Paraffin-Embedded Tissue by Multiplex Ligation-Dependent Probe Amplification.- Application of Microarrays for DNA Methylation Profiling.- Genome-Wide Identification of Protein Binding Locations using Chromatin Immunoprecipitation coupled with Microarray.- Transcriptional Profiling of Small Samples in the Central Nervous System.
- ISBN: 978-1-58829-871-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 530
- Fecha Publicación: 01/03/2008
- Nº Volúmenes: 1
- Idioma: Inglés