Provides detailed guidelines for molecular analysis of archive tissuesAn invaluable aid for researchers and pathologists involved in translational researchand diagnosticsCaters for the needs of the less experienced through the inclusion of clear notes and explanationsWritten by experts who have developed the described methods and validated them within the framework of a multi-institution European projectCaters for the needs of the less experienced through the inclusion of clear notes and explanationsWritten by experts who have developed the described methods and validated them within the framework of a multi-institution European projectWritten by experts who have developed the described methods and validated them within the framework of a multi-institution European project INDICE: Introduction. Archive Tissues. Pre-Analytical Treatment of Tissues. Dissection of Tissue Components. Extraction, Purification, Quantification and Quality Assessment of Nucleic Acids. Reverse Transcription (RT). Nucleic Acid Amplification. DNA Sequencing. Microsatellite Analysis. Methylation Analysis. Analysis of Copy Number Variations. Microarrays. Protein Extraction in Archive Tissues. Protein Electrophoretic Separation. Protein Immunodetection. Mass Spectrometry. Elements of Good Laboratory Practice. DNA Sequencing. Microsatellite Analysis. Methylation Analysis. Analysis of Copy Number Variations. Microarrays. Protein Extraction in Archive Tissues. Protein Electrophoretic Separation. Protein Immunodetection. Mass Spectrometry. Elements of Good Laboratory Practice.
- ISBN: 978-3-642-17889-4
- Editorial: Springer Berlin Heidelberg
- Encuadernacion: Cartoné
- Páginas: 180
- Fecha Publicación: 01/07/2011
- Nº Volúmenes: 1
- Idioma: Inglés