Given the popularity and utility of Saccharomyces cerevisiae, yeast-based functional genomics and proteomics technologies, developed over the past decade, have contributed greatly to our understanding of bacterial, yeast, fly, worm and human gene functions. In Yeast Functional Genomics and Proteomics: Methods and Protocols, experts in the field contribute stand-alone protocols suitable for daily use in research laboratories. The volume examines methods from the most major and fundamental techniques to more cutting-edge, advanced concepts. As part of the highly successful Methods in Molecular Biology™ series, the chapters are clearly formatted with introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproduciblelaboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Covers a wide range of state-of-the-art yeast-based functional genomics andproteomics technologies Provides an easily accessible reference volume for most important yeast-based functional genomics and proteomics technologies written by leading researchers in the field Comprehensive guide to running and analyzing yeast-based assays in functional genomics and proteomics INDICE: From the contents 1. Comparative Genome Hybridization on Tiling Microarrays to Detect Aneuplodies in Yeast. 2. Identification of Transcription Factor Targets by Phenotypic Activation and Microarray Expression Profiling in Yeast. 3. SGAM, an Array-based Approach for High-Resolution Genetic Mapping inSaccharomyces cerevisiae. 4. Reporter-based Synthetic Genetic Array Analysis:A Functional Genomics Approach for Investigating the Cell Cycle in Saccharomyces cerevisiae. 5. The Fidgety Yeast: Focus on High-Resolution Live Yeast CellMicroscopy. 6. A Genomic Approach to Yeast Chronological Aging. 7. Chemogenomic Approaches to Elucidation of Gene Function and Genetic Pathways. 8. Identification of Inhibitors of Chromatin Modifying Enzymes Using the Yeast Phenotypic Screens. 9. Exploiting Yeast Genetics to Inform Therapeutic Strategies for Huntington’s Disease. 10. Global Proteomic Analysis of S. cerevisiae (GPS) Identifies Molecular Pathways of Histone Modifications.
- ISBN: 978-1-934115-71-8
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 280
- Fecha Publicación: 01/05/2009
- Nº Volúmenes: 1
- Idioma: Inglés