Over the past decades, the pathogenesis, diagnosis, treatment and prevention of cardiovascular diseases have been benefited significantly from intensive research activities. In order to provide a comprehensive ‘manual’ in a field that has become as broad and deep as cardiovascular medicine, this volume of ‘Methods in Molecular Medicine’ covers a wide spectrum of in vivo and in vitro techniques encompassing biochemical, pharmacological and molecular biology disciplines which are currently used to assess vascular disease progression. Each chapter included in this volume focuses on a specific vascular biology techniqueand describes various applications as well as caveats of these techniques. The protocols included here are described in detail, allowing beginners with little experience in the field of vascular biology to embark on new research projects. Includes new updates on traditional methods Provides various methodologies for gene therapy and gene-knockout Highly specific, easy to follow step-by-step laboratory protocols INDICE: From the contents Rat Carotid Artery Balloon Injury Model.- Histological and Morphometric Analyses for Rat Carotid Artery Balloon Injury.- Plaque Rupture Model in Mice.- Immunostaining of Mouse Atherosclerotic Lesions.- Surgical Animal Model of Ventricular Hypertrophy.- Animal Models of Hypertension.- Rat Models of Cardiac Insulin Resistance.- The Isolated, Perfused Pseudo-Working Heart Model.- Altering and Analyzing Glucose Metabolism in Perfused Hearts of Transgenic Mice.- Methods in Evaluation of Cardiovascular Renin Angiotensin Aldosterone Activation and Oxidative Stress.- Detection and Quantificationof Apoptosis in the Vasculature.- Endothelial Progenitor Cells and Mesenchymal Stem Cell Isolation, Characterization and Viral Transduction.- Assessment ofEndothelial Damage/Dysfunction: A Focus on Circulating Endothelial Cells.- Evaluation of Endothelial Function by In-Vivo Microscopy.- Haptotaxis of Endothelial Cell Migration under Flow.
- ISBN: 978-1-58829-574-3
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 372
- Fecha Publicación: 01/10/2008
- Nº Volúmenes: 1
- Idioma: Inglés