Single-Molecule Enzymology: Nanomechanical Manipulation and Hybrid Methods

Single-Molecule Enzymology, Part B, the latest volume in the Methods in Enzymology series, continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers research methods in single-molecule enzymology, and includes sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion. Continues the legacy of this premier serial with quality chapters authored by leaders in the fieldCovers research methods in single-molecule enzymologyContains sections on such topics as force-based and hybrid approaches, fluorescence, high-throughput sm enzymology, and nanopore and tethered particle motion INDICE: Single-molecule optical trapping techniques to study molecular mechanisms of a replisomeMichelle D. WangHow to measure load-dependent kinetics of individual motor molecules without a force clampHenrik Flyvbjerg, James Spudich, Kim Mortensen and Jongmin Sung Studying the mechanochemistry of processive cytoskeletal motors with an optical trapAhmet YildizRecent advances in biological single-molecule applications of optical tweezers and fluorescence microscopyErwin Peterman, Gijs Wuite and Maryam Hashemi Shabestari Combining single-molecule technologies: DNA tightropes and optical trapping of complex micro-structuresNeil Kad and Jingyu Wang Direct Visualization of Helicase Dynamics using Fluorescence Localization and Optical TrappingTaekjip HaHigh-resolution optical tweezers combined with single-molecule confocal microscopyYann R. ChemlaSingle-stranded DNA curtains for studying homologous recombinationEric C. GreeneInserting extrahelical structures into long DNA substrates for single-molecule studies of DNA mismatch repairIlya J. FinkelsteinSingle-molecule insight into target recognition by CRISPR-Cas complexesRalf SeidelSingle-molecule enzymologyH. Peter LuMT - helicases/replicationVincent CroquettePreparation of DNA substrates and functionalized glass surfaces for correlative nanomanipulation and colocalization (NanoCOSM) of single moleculesTerence StrickMeasuring force induced dissociation kinetics of protein complexes using single molecule atomic force microscopySanjeevi SivasankarImproved force spectroscopy using focused-ion-beam modified cantileversThomas T. Perkins and Jaevyn K. Faulk Single-molecule characterization of DNA-protein interactions using nanopore biosensorsAmit MellerSubangstrom measurements of enzyme function using a biological nanopore, SPRNTJens H. GundlachMultiplexed, tethered particle microscopy for studies of DNA-enzyme dynamics Laura Finzi

• ISBN: 978-0-12-809310-8
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 412
• Fecha Publicación: 10/01/2017
• Nº Volúmenes: 1
• Idioma: Inglés