With the growing global fear of a major pandemic, avian influenza virus research has increased greatly in importance during this young century. In Avian Influenza Virus, an expert team of researchers and diagnosticians examine the fundamental yet essential virological methods for AI virus research and diagnostics as well as some of the newest molecular procedures currently used for basic and applied research. Exciting, cutting-edge new methods focus on studying the virus itself and work with avian hosts, an area greatly lacking in research. Following the format of the highly successful Methods in Molecular Biology™ series, each chapter provides readily reproducible laboratory protocols offering step-by-step instruction and lists of the necessary equipment for the task.Comprehensive and timely, Avian Influenza Virus equips diagnosticians and researchers with the current tools and information they need to learn more about this high impact disease. Contains protocols for virus detection, isolation and propagation, and virus characterization. Includes cellular immunological methods and immunohistochemistry with a focus on avian hosts. Covers reverse genetics with avian influenza virus. Details wild bird monitoring including sampling methods and virus detection. INDICE: From the contents A brief introduction to avian influenza virus.- Avian Influenza Virus Sample Types, Collection, and Handling.- Avian influenzavirus RNA extraction from tissue and swab material.- Type-A Influenza Virus Detection and Quantitation by Real-time RT-PCR.- Detection and Identification of the H5 Hemagglutinin subtype by Real-time RT-PCR.- Avian influenza virus isolation and propagation in chicken eggs.- Hemagglutination Assay for Avian Influenza Virus.- Hemagglutination-Inhibition Test for Avian Influenza Virus Subtype Identification and the Detection and Quantititation of Serum Antibodies to Avian Influenza Virus.- Neuraminidase-Inhibition Assay for the Identification of Influenza A Virus Neuraminidase Subtype or Neuraminidase Antibody Specificity.- Immunohistochemical Staining for the Detection of Avian Influenza Virus in Tissues.- Wild bird Surveillance for Avian Influenza Virus.- Reverse Genetics of Avian Influenza Virus.
- ISBN: 978-1-58829-939-0
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 230
- Fecha Publicación: 01/03/2008
- Nº Volúmenes: 1
- Idioma: Inglés