The rise of molecular biology now permits a clear examination of the genetic basis for various hearing and balance disorders. In Auditory and Vestibular Research: Methods and Protocols, specialists in the field describe current RNA, protein, and imaging protocols that have provided insights into genetic regulation as well as a greater understanding of the genes and pathogens involved indiseases of the ear. This overview utilizes both mammalian and non-mammalian animal models as well as techniques applicable to clinical studies in order tobest provide an up-to-date perspective on basic research. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls. Lists all the reagents, step-by-step techniques, and limitations for reproducible results in DNA/RNA tracing, protein identification, and microscopic imaging Clearly describes the protocols necessary for in vivo and in vitro gene delivery techniques Provides the tools to identify and isolate cochlear stem cells for the treatment of inner ear pathologies INDICE: From the contents RNA Isolation from Xenopus Inner Ear Sensory Endorgans for Transcriptional Profiling and Molecular Cloning.- Synthesis of Biotin-Labeled RNA for Gene Expression Measurements Using Oligonucleotide Arrays.-In situ Hybridization Approach to Study mRNA Expression and Distribution in Cochlear Frozen Sections.- Lineage Analysis of Inner Ear Cells Using Genomic Tags for Clonal Identification.- Genetic Fate Mapping Approaches: New Means to Explore the Embryonic Origins of the Cochlear Nucleus.- The Practical Use of Cre and loxP Technologies in Mouse Auditory Research.- Helios® Gene Gun-MediatedTransfection of the Inner Ear Sensory Epithelium.- Electroporation-Mediated Gene Transfer to the Developing Mouse Inner Ear.- Isolation of Sphere-Forming Stem Cells from the Mouse Inner Ear.- Molecular Biology of Vestibular Schwannomas.- Multilocus Sequence Typing and Pulsed-Field Gel Electrophoresis of OtitisMedia Causing Pathogens.
- ISBN: 978-1-934115-62-6
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 370
- Fecha Publicación: 01/11/2008
- Nº Volúmenes: 1
- Idioma: Inglés