Serum/plasma proteomics: methods and protocols

with its well-honed methodologies, seeks to serve both professionals and investigators new to the field in an effort to further our knowledge of this fundamental science. Includes cutting-edge methods and protocols. Provides step-by-step detail essential for reproducible results. Contains key notes and implementation advice from experts. INDICE: Fractionation Strategies for In-depth Blood Proteome Analysis. Plasma Biomarker Discovery using 3-D Protein Profiling Coupled with Label-free Quantitation. Intact Protein Separation by 1 and 2-dimension Liquid Chromatography for Comparative Proteomic Separation of Partitioned Serum or Plasma. In-depth Analysis of a Plasma or Serum Proteome using a 4-D Protein Profiling Method. Intact-protein Analysis System (IPAS) for Discovery of Serum-based Disease Biomarkers. Model-based Discovery of Circulating Biomarkers. Low-molecular Weight Plasma Proteome Analysis using Centrifugal Ultrafiltration. High-throughputAnalysis of Glycoproteins from Plasma. Blood Collection and Handling Strategies. Minimizing Pre-analytical Variation of Plasma Samples by Proper Blood Collection and Handling. Collection and Handling of Blood Specimens for Peptidomics. Investigation of Peptide Biomarker Stability in Plasma Samples using Time-course MS Analysis. Methods for Quantitative Assaying of Blood-Based Biomarkers. Biomarker Validation in Blood Specimens by Selected Reaction Monitoring MassSpectrometry of N-glycosites. A Fluorescent Microsphere-based Method for Assay of Multiple Analytes in Plasma. Immuno-mass Spectrometry: Quantification of Low Abundance Proteins in Biological Fluids. Qualitative and Quantitative Analysis of Glycated Proteins in Human Plasma by Glucose Isotopic Labeling with 13C 6-reducing Sugars. Proteome Analysis of Blood Cell Components, Vesicles, and Blood-related Fluids. Exosome Isolation for Proteomic Analyses and RNA Profiling. Extraction and Proteome Analysis of Liver Tissue Interstitial Fluid. A Protocol for the Preparation of Cryoprecipitate and Cryo-depleted Plasma. Preparation of Platelet Concentrates. Phosphoproteome Analysis of the PlateletPlasma Membrane. Bioinformatic Analysis of Blood Proteins and Peptides. Statistical Design and Analysis of Label-free LC-MS Proteomic Experiments: A Case Study of Coronary Artery Disease. Data Management in Mass Spectrometry-based Proteomics. Bioinformatics Challenges in the Proteomic Analysis of Human Plasma.Using the Human Plasma PeptideAtlas to Study Human Plasma Proteins.

• ISBN: 978-1-61779-067-6
• Editorial: Humana Press
• Encuadernacion: Cartoné
• Páginas: 350
• Fecha Publicación: 29/04/2011
• Nº Volúmenes: 1
• Idioma: Inglés