This new edition offers a comprehensive guide to cutting-edge methods used today across the field of molecular embryology. These powerful techniques take advantage of the most commonly used vertebrate experimental models: murine embryos for their genetics, chick embryos for in vivo manipulation, zebrafish for mutagenesis, amphibian embryos, and nonvertebrate chordates. The book emphasizes the most important methods of experimental molecular biology and the particular advantages of each different species. Provides an excellent source for understanding the strengths and weaknesses of the various vertebrate model organisms Comprehensive guide to the cutting-edge methods amongst the dramatically growing field of vertebrate molecular embryology Experts in the field thoroughly describe protocols and techniques accompanied by illustrations New approaches for efficient production of transgenic zebrafish and microarrays INDICE: From the contents The Mouse as a Developmental Model.- Culture of Postimplantation Mouse Embryos.- Organ Culture in the Analysis of Tissue Interactions.- Treatment of Mice with Retinoids In Vivo and In Vitro.- Analysis of Skeletal Ontogenesis through Differential Staining of Bone and Cartilage.- Cell Grafting and Labeling in Postimplantation Mouse Embryos.- Production of Transgenic Rodents by the Microinjection of Cloned DNA Into Fertilized One-Celled Eggs.- Cre Recombinase Mediated Alterations of the Mouse Genome Using Embryonic Stem Cells.- Gene Trapping in Mouse Embryonic Stem Cells.- Application of lacZ Transgenic Mice to Cell Lineage Studies.- Transgenic RNA Interference to Investigate Gene Function in the Mouse.- Mouse Primordial Germ Cells: Isolation and In Vitro Culture.- Gene Transfer to the Rodent Embryo by Retroviral Vectors.- The Avian Embryo: An Overview.- Chick Embryos: Incubation and Isolation.- New Culture.- EC –culture: a method to culture early chick embryos.
- ISBN: 978-1-58829-431-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 400
- Fecha Publicación: 01/08/2008
- Nº Volúmenes: 1
- Idioma: Inglés