MALDI-TOF and Tandem MS for Clinical Microbiology

This book highlights the triumph of MALDI–TOF mass spectrometry over the past decade and provides insight into new and expanding technologies through a comprehensive range of short chapters that enable the reader to gauge their current status and how they may progress over the next decade.  This book serves as a platform to consolidate current strengths of the technology and highlight new frontiers in tandem MS/MS that are likely to eventually supersede MALDI–TOF MS. Chapters discuss:Challenges of Identifying Mycobacterium to the Species level Identification of Bacteroides and Other Clinically Relevant AnaerobesIdentification of Species in Mixed Microbial PopulationsDetection of Resistance MechanismsProteomics as a biomarker discovery and validation platformDetermination of Antimicrobial Resistance using Tandem Mass Spectrometry INDICE: Preface .MALDI–TOF Mass Spectrometry .1. MALDI–TOF and LC–MS/MS: A Paradigm Shift from Research to Frontline Microbial Diagnostics; A Laboratory?s Vision and Relentless Resolve to Help Develop and Implement this New Technology Amidst Formidable Obstacles .2. Criteria for Development of MALDI–TOF Mass Spectral Database .3. Applications of MALDI–TOF Mass Spectrometry in Clinical Diagnostic Microbiology .4. The Challenges of Identifying Mycobacterium to the Species Level using MALDI–TOF MS .Section A: Modifications of Standard Bruker Biotyper Method .Section B: ASTA s MicroID System and its MycoMp Database for Mycobacteria .5. Transformation of Anaerobic Microbiology since the Arrival of MALDI–TOF–MS .6. The Potential of MALDI–TOF MS to Distinguish between Taxonomically Similar Species that Manifest Different Infections .7. Identification of Species in Mixed Bacterial Populations using MALDI–TOF MS .8. Microbial DNA Analysis byMALDI–TOF Mass Spectrometry .Section A:DNA Analysis of Viral Genomes using MALDI–TOF MS .Section B:Mass Spectral Analysis of Proteins of Nonculture and Cultured Viruses .9. Analysis of Yeast and Fungi using a Non–Commercial Database .10. Development and Application of MALDI–TOF MS for Detection of Resistance Mechanisms .11. Discrimination of Burkholderia, Brucellaspp and other Taxa at the Subspecies Level Using MALDI–TOF Mass Spectrometry .12. MALDI–TOF–MS Based Upon the Ribosomal Proteins Coding in S10 for Proteotyping .Tandem MS/MS based Approaches to Microbial Characterisation .13. Tandem Mass Spectrometry Analysis as an Approach to Delineate Genetically Related Taxa .14. Mapping of the Proteogenome of Clostridium difficile Isolates of Varying Virulence .15. Determination of Antimicrobial Resistance using Tandem Mass Spectrometry .16. Proteotyping: Tandem Mass Spectrometry and Proteomic Analysis of Pathogenic Microorganisms .17. Proteogenomics of Pseudomonas aeruginosa in Chronic Cystic Fibrosis Infections .18. Top–Down Proteomics in the Study of Microbial Pathogenicity .19. Tandem Mass Spectrometry in Resolving Complex Gut Microbiota Functions .20. Proteogenomics of Non–Model Microorganisms .21. Analysis of Mass Spectral Data for Species Identification and Typing of Microorganisms .Section A:The analysis of MALDI–TOF MS spectra using the BioNumerics software .Section B:Subtyping of Staphylococcus spp. based upon MALDI–TOF MS Data .Section C: Elucidating the Intra–Species Proteotypes of Pseudomonas aeruginosa from Cystic Fibrosis

• ISBN: 978-1-118-96025-7
• Editorial: Wiley–Blackwell
• Encuadernacion: Cartoné
• Páginas: 648
• Fecha Publicación: 09/06/2017
• Nº Volúmenes: 1
• Idioma: Inglés