This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. Circadian Rhythms and Biological Clocks is an exceptional resource for anybody interested in the general area of circadian rhythms. As key elements of timekeeping are conserved in organisms across the phylogenetic tree, and our understanding of circadian biology has benefited tremendously from work done in many species, the volume provides a wide range of assays for different biological systems. Protocols are provided to assess clock function, entrainment of the clock to stimuli such as light and food, and output rhythms of behavior and physiology. This volume also delves into the impact of circadian disruption on human health. Contributions are from leaders in the field who have made major discoveries using the methods presented here. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers research methods in biomineralization scienceKeeping with the interdisciplinary nature of the circadian rhythm field, the volume includes diverse approaches towards the study of rhythms, from assays of biochemical reactions in unicellular organisms to monitoring of behavior in humans. INDICE: 1. Genetic screens for circadian and sleep mutants in Drosophila2. Dissecting mechanisms of the clock in Neurospora3. High throughput and quantitative approaches for measuring circadian rhythms in cyanobacteria using bioluminescence4. Measuring rhythms in worms5. Wavelet-based analysis of circadian behavioral rhythms6. Post-translational regulation of clock proteins7. The role of casein kinase 1 in the circadian clock8. Purification and analysis of the mammalian circadian clock PERIOD complex. 9. Structures of clock proteins10. Identification of small molecule modulators of clocks11. Analysis of the redox clock and its interaction with the transcription-translation feedback loop12. Measuring synchrony in the mammalian central clock circuit13. Clocks and neuronal function14. Analysis of the clock circuit in Drosophila15. Evaluating the role of glia in circadian regulation16. Seasonal and age-dependent changes in the SCN neural network17. Photic entrainment in Drosophila18. Response of peripheral rhythms to the timing of food intake in mammals19. Photic regulation of clock systems20. Circadian control of transcription 21. Profiling rhythms of gene expression22. Profiling gene expression across the clock circuit23. Control of circadian gene expression by post-transcriptional mechanisms 24. ChIPping away at the Drosophila clock25. Circadian regulatory networks in plants26. Genetic approaches to the study of natural variation in circadian behavior27. Determining the adaptive value of circadian rhythms28. Circadian regulation of Cardiovascular Biology29. Circadian regulation of cellular physiology30. Genetics of human circadian/sleep disorders31. Measuring circadian clock function in human cells and matrices32. Sleep:wake cycles and mental health33. Phenotyping of cognitive vulnerability to circadian phase during sleep loss34. Variations in sleep across human subjects35. Detecting KaiC phosphorylation rhythms of the cyanobacterial circadian oscillator in vitro and in vivo36. Best practices for fluorescence microscopy of the cyanobacterial circadian clock
- ISBN: 978-0-12-801218-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 514
- Fecha Publicación: 15/01/2015
- Nº Volúmenes: 1
- Idioma: Inglés