Laboratory Methods in Microbiology and Molecular Biology: Methods in Molecular Microbiology

Laboratory Methods in Microbiology and Molecular Biology: Methods in Molecular Microbiology is a unique, informative and multidisciplinary work that is not only confined to one discipline. It provides various procedures in diverse disciplines from microbiology to genetics, molecular biology and biochemistry. Here, the principles and facts underlying practical applications of bacteria and fungi which have prospects in various technologies are listed. It is composed of 15 chapters broadly covering basic concepts in microbiology, including soil microbiology, food microbiology, microbial genetics, serology and the use of basic bioinformatics tools. Each chapter contains a set of experiments with an introduction, principle, reagents and buffers used, brief methodology, conclusions and inferences, and a list of 20-40 references. A brief summary/abstract is also included. The book will facilitate students, teachers and researchers to monitor the precision and accuracy of their qualitative and quantitative methods practically. Involves various procedures in diverse disciplines, from microbiology to genetics, molecular biology and biochemistry Lists the principles and facts underlying practical applications of bacteria and fungi which have prospects in various technologies Includes the questions 'how' and 'why' as an explanation for novice students and researchers to modify protocols Facilitates students, teachers and researchers to monitor the precision and accuracy of their qualitative and quantitative methods practically INDICE: General laboratory notes Basics in techniques: Micropipeting, Weighing Preparation of stock solutions, stains, buffers and reagents, indicators Basic concepts in Microbiology i. Preparation of culture media ii. Isolation and purification of bacteria (spreading, streaking, sub-culturing) iii. Preservation methods of bacteria and fungi Microscopy and imaging analysis i. Wet mount technique ii. Hanging drop experiment iii. Preparation of samples and smears iv. Simple staining v. Negative staining vi. Gram staining vii. Special staining- capsule, spore DNA staining Bacterial physiology i. Physiological characterization of bacteria ii. Effect of temperature iii. Effect of pH iv. Effect of salt concentration Preservation methods and maintenance of cultures Soil Microbiology Isolation of Rhizobium Isolation of Azotobacter Isolation of Actinomycetes Direct confrontation test Food Microbiology Isolation of lactic acid bacteria Microbiological analysis of various food products MBRT to test milk quality Preparation of yogurt Preparation of bread Basic experiments in Microbial Biochemistry Biochemical tests Plate assays for the detection of enzymes viz proteases, cellulases, amylase etc Techniques in Microbial Genetics i. Conjugation ii. Transformation iii. Antibiotic sensitivity testing Analytical techniques in Molecular Biology i. DNA isolation from soil sample ii. DNA isolation from Bacteria iii. DNA isolation from fungi iv. RNA isolation from bacteria and fungi v. Agarose gel electrophoresis vi. Polymerase chain reaction vii. Ligation viii. Preparation of competent cells ix. Cloning and Transformation Blue white screening x. Plasmid isolation using alkaline lysis method xi. Restriction digestion both single and double digestion xii. Cloning and transformation in expression vector xiii. Expression of recombinant protein in heterologous host xiv. SDS-PAGE xv. In gel assay xvi. Protein estimation Bradford assay xvii. Enzyme assays xviii. Western Blot analysis Sero-diagnostics i. Estimation of RBCs using Haemocytometer ii. Estimation of blood groups iii. Agar gel precipitation test iv. Ochterlony double diffusion test v. Radio immune assay vi. Sandwich ELISA vii. Immunoelectrophoresis viii. DOT ELSIA ix. Widal test Bioinformatic methods and protocols Analyzing microbes

• ISBN: 978-0-323-95078-7
• Editorial: Academic Press
• Encuadernacion: Rústica
• Páginas: 280
• Fecha Publicación: 01/03/2023
• Nº Volúmenes: 1
• Idioma: Inglés