In recent decades, infectious diseases, once believed to be fairly contained,have become a vital, resurgent field of research. In Host-Pathogen Interactions: Methods and Protocols, top experts examine the relationship between the host and the pathogen, crucial in the outcome of an infection and the establishment of disease or asymptomatic, commensal colonization by organisms. The step-by-step laboratory methods and protocols of this volume study host-pathogen interaction, with a focus on fungal, bacterial and parasitic pathogens, at a molecular level in order to reveal the mechanisms of infection and to identify the vulnerabilities of the pathogen of interest. Written in the highly successful Methods in Molecular Biology™ series format, the chapters feature brief subject introductions, lists of the necessary materials and reagents, and tips on troubleshooting and avoiding known pitfalls. Ready-to-start protocols established for reliability by experts in the field Easy to reproduce Comprehensive coverage of the models of infection and methods to study the major human pathogenic bacteria, fungi as well as parasites INDICE: From the contents Introduction: Bacterial Pathogens.- Transcription Analyses in the Interaction of Neisseria meningitidis with Mammalian Host Cells.- Surface-exposed adherence molecules of Streptococcus pneumoniae.- Screening of Virulence Traits in Legionella pneumophila and Analysis of the Host Susceptibility to Infection by Using the Dictyostelium Host Model System.- Characterizing Host Receptor Recognition by Individual Bacterial Pathogens.- Introduction: Fungal Pathogens.- Isolation and Purification of Antigenic Components of Cryptococcus.- A Method for Examining Glycans Surface Expression of Yeasts by Flow Cytometry.- Human Epithelial Model Systems for the Study of Candida Infections in vitro: Part I. Adhesion to Epithelial Models.- Human Epithelial Model Systems for the Study of Candida Infections in vitro: Part II. HistologicalMethods for Studying Fungal Invasion.- In vitro Systems for Studying the Interaction of Fungal Pathogens with Primary Cells from the Mammalian Innate Immunity.
- ISBN: 978-1-58829-886-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 370
- Fecha Publicación: 01/09/2008
- Nº Volúmenes: 1
- Idioma: Inglés