In light of the critical contributions of macrophages and dendritic cells to diverse inflammatory diseases and to immunity and host defense, state-of-the-art approaches to the investigation of their behavior are essential. In Macrophages and Dendritic Cells: Methods and Protocols, expert researchers contributelaboratory protocols involving these two vital cell types functioning at the junction of the innate and acquired immune systems. The volume delves first into isolation and cell culturing then continues with topics such as phagocytosis, genetic manipulation, macrophage activation, and lipid signaling. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Provides an easily accessible reference volume for state-of-the art experimentation by leading researchers in the field Covers a wide range of approaches for the isolation and characterization of macrophages and dendritic cells Provides, detailed step-by-stepprotocols to modify gene expression in cells that are notoriously resistant to genetic manipulation INDICE: From the contents Generation of Retroviral Macrophage cDNA Expression Libraries and Functional Screening for Surface Receptors.- Generation of Murine Growth Factor-Dependent Long-Term Dendritic Cell Lines to Investigate Host-Parasite Interactions.- Macrophage Activation: Classical Versus Alternative.- Analysis of Macrophage Phagocytosis: Quantitative Assays of Phagosome Formation and Maturation Using High-Throughput Fluorescence Microscopy.- Detection of Activated Rab7 GTPase with an Immobilized RILP Probe.- Probing In Vivo Origins of Mononuclear Phagocytes by Conditional Ablation and Reconstitution.- Multivalent Protein Probes for the Identification and Characterization of CognateCellular Ligands for Myeloid Cell Surface Receptors.- Exploiting Fc ChimaericProteins for the Identification of Ligands Specific for the Mannose Receptor.- The Expression of Exogenous Genes in Macrophages: Obstacles and Opportunities.- Genetic Modification of Murine Dendritic Cells by RNA Transfection.
- ISBN: 978-1-58829-972-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 390
- Fecha Publicación: 01/03/2009
- Nº Volúmenes: 1
- Idioma: Inglés