Given the versatile utility of the determinination of epitopes, beneficial toa wide variety of scientists from immunologists to structural biologists to biotechnologists, the need for a thorough, state-of-the-art collection of experimental protocols is clear. In Epitope Mapping Protocols, Second Edition, expert contributors from a broad spectrum of scientific backgrounds update and expand the successful first edition with cutting-edge techniques and applications, including approaches to both antibody or B-cell epitope mapping and T-cell epitope mapping as well as a new section on the profiling of antibody signatures in biological fluids. Written in the popular Methods in Molecular Biology™ series format, chapters include brief introductions to the topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, which highlight tips on troubleshooting and avoiding known pitfalls. Provides an easily accessible reference volume for epitope mapping written by leading researchers in the field Details the process of turning epitope mapping into biomarker discovery Covers a wide range of antibody-based and T-cell-based assay formats and systems Presents a modular approach to experimental design, screening modalities, detection technologies, andapproaches to data analysis INDICE: From the contents What Is a B Cell Epitope?- Structural Basis of Antibody-Antigen Interactions.- Epitope Mapping of Antibody-Antigen Complexes by Nuclear Magnetic Resonance Spectroscopy.- A Solid-Phase Mutual Inhibition Assay with Labeled Antigen.- Epitope Mapping by Surface Plasmon Resonance.- Proteolytic Fragmentation for Epitope Mapping.- Epitope Mapping by Proteolysis of Antigen – Antibody Complexes.- Identifying Residues in Antigenic Determinants by Chemical Modification.- Epitope Mapping by Differential Chemical Modification of Antigens.- Linear B-Cell Epitope Mapping Using Enzyme-Linked Immunosorbent Assay for Libraries of Overlapping Synthetic Peptides.- Antibody Epitope Mapping Using SPOTTM Peptide Arrays.- Peptide Microarrays for Profiling of Modification State Specific Antibodies.- Epitope Mapping Using Phage Display Peptide Libraries.- Antibody Epitope Mapping Using De Novo Generated Synthetic Peptide Libraries.- Antibody Specificity Profiling on Functional Protein Microarrays.
- ISBN: 978-1-934115-17-6
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 400
- Fecha Publicación: 01/03/2009
- Nº Volúmenes: 1
- Idioma: Inglés