Biophysical Approaches for the Study of Membrane Structure Part A

Biophysical Approaches for the Study of Membrane Structure, Part A, Volume 700 explores lipid membrane asymmetry and lateral heterogeneity. A burst of recent research has shown that bilayers whose leaflets differ in their physical properties-such as composition, phase state, or lateral stress-exhibit many fascinating new characteristics, but also pose a host of new challenges related to their creation, characterization, simulation, and theoretical description. Chapters in this new release include Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCS, Effects of lateral and hydrostatic pressure on membrane structure and properties, and much more.Other sections cover Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cells, Direct quantification of cellular membrane lipids using ratiometric fluorescence sensors, The spectral phasor approach to resolving membrane order with environmentally sensitive dyes, The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domains, Advanced microscopy methods to study membrane pores, Use of cryo-EM to study membrane phase separation, and much more. Explore the state-of-the-art of lipid membrane asymmetryCovers experimental, theoretical, and computational techniques to create and characterize asymmetric lipid membranesTeaches how these kinds of approaches create and characterize laterally inhomogeneous membranes INDICE: 1. Evaluation of functional transbilayer coupling in live cells by controlled lipid exchange and imaging FCSNirmalya Bag, Arpita Tripathy and Sudipti Priyadarsinee2. Fluorescence imaging of lamellipodin mediated biomolecular condensates on solid supported lipid bilayer membranesTobias Baumgart3. Effects of lateral and hydrostatic pressure on membrane structure and propertiesNick Brooks4. Using the yeast vacuole as a system to test the lipid drivers of membrane heterogeneity in living cellsItay Budin, Israel Juarez-Contreras and Hyesoo Kim5. Direct quantification of cellular membrane lipids using ratiometric fluorescence sensorsWonhwa Cho6. The spectral phasor approach to resolving membrane order with environmentally sensitive dyesRumiana Dimova and Agustín Mangiarotti7. The use of hemifusion to create asymmetric giant unilamellar vesicles: Insights on induced order domainsThais A. Enoki8. Advanced microscopy methods to study membrane poresAna J. García-Sáez9. Use of cryo-EM to study membrane phase separationFrederick Heberle and M. Neal Waxham10. Dark secrets of substrate preparation for patterned supported bilayer experimentsAurelia Honerkamp-Smith11. Using lipid binding proteins to study lipid membrane heterogeneityTOSHIHIDE KOBAYASHI12. Structural characterization of lateral phase separation in polymer-lipid hybrid membranesCecília Leal, Nurila Kambar, Minh N. Do, Corey Snyder and Yoo Kyung Go13. Applications of phase-separating multi-bilayers in protein binding/partitioning/signaling and membrane-associated condensate formationKandice R. Levental and Hong-Yin Wang14. Studying lipid flip-flop using NMR and TR-SANSDrew Marquardt, Dominik Dziura and Maksymilian Dziura15. Measuring bilayer thickness from cryo-TEM of hydroperoxidized POPC and SOPCCarlos Marques and André Schroder16. Structure of symmetric and asymmetric lipid membranes from joint SAXS/SANSGeorg Pabst, Enrico Semeraro and Moritz Frewein17. Quantification of membrane geometry and protein sorting on cell membrane protrusions using fluorescence microscopyZheng Shi18. A TIRF microscopy-based method for observing the uptake of individual particles by clathrin mediated endocytosisJeanne Stachowiak, Grant Ashby, Kayla Keng and Carl Hayden19. Pore-spanning membranes as a tool to investigate lateral lipid membrane heterogeneityClaudia Steinem and Larissa Socrier20. MaCD based plasma membrane outer leaflet lipid exchange in mammalian cells to study insulin receptor activityPavana Suresh and Erwin London

• ISBN: 978-0-443-29304-7
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Páginas: 534
• Fecha Publicación: 01/08/2024
• Nº Volúmenes: 1
• Idioma: Inglés