Mesenchymal stem cells: methods and protocols

For over forty years, mesenchymal stem cells (MSCs) have been scrutinized andstudied, garnering much attention due to their broad therapeutic efficacy. InMesenchymal Stem Cells: Methods and Protocols, leaders in the field were assembled to contribute detailed methodologies for the isolation and characterization of human and rodent MSCs. Recently, these vital cells have shown therapeutic benefits in the treatment of myocardial infarction, stroke, lung diseases, spinal cord injury and other neurological disorders, thus promising a boundless future in their study. Cutting edge and easy to use, Mesenchymal Stem Cells:Methods and Protocols is the perfect resource for scientists attempting to pursue this important and ever-developing field of research. An essential guide to MSC research for both newcomers and established researchers in the field. Contains chapters on isolation and expansion of mouse and human stem cells. Explores stem cells from bone marrow and adipose tissue. Provides procedures for differentiation of stem cells along multiple lineages. INDICE: Isolation and culture of bone marrow-derived human multipotent stromal cells (hMSCs). Mesenchymal stem cells from adult bone marrow. A method toisolate and purify human bone marrow stromal stem cells. Adipose-derived stemcells. Isolation of human adipose-derived stem cells from biopsies and liposuction specimens. Colony forming unit assays for MSCs. Differentiation and characterization of human MSCs. Freezing harvested hMSCs and recovery of hMSCs from frozen vials for subsequent expansion, analysis and experimentation. Gene expression analysis at the single cell level using the human bone marrow stromalcell as a model: sample preparation methods. Assays of MSCs with microarrays.Gene delivery to mesenchymal stem cells. Isolation of mesenchymal stem cells from murine bone marrow by Immunodepletion.

• ISBN: 978-1-58829-771-6
• Editorial: Humana
• Encuadernacion: Cartoné
• Páginas: 220
• Fecha Publicación: 01/05/2008
• Nº Volúmenes: 1
• Idioma: Inglés