Numerical methods for the life scientist: binding and enzyme kinetics calculated with GNU octave and MATLAB

Enzyme kinetics, binding kinetics and pharmacological dose-response curves are currently analyzed by a few standard methods. Some of these, like Michaelis-Menten enzyme kinetics, use plausible approximations, others, like Hill equations for dose-response curves, are outdated. Calculating realistic reaction schemes requires numerical mathematical routines which usually are not covered inthe curricula of life science. This textbook will give a step-by-step introduction to numerical solutions of non-linear and differential equations. It willbe accompanied with a set of programs to calculate any reaction scheme on anypersonal computer. Typical examples from analytical biochemistry and pharmacology can be used as versatile templates. When a reaction scheme is applied fordata fitting, the resulting parameters may not be unique. Correlation of parameters will be discussed and simplification strategies will be offered. First advanced text for life scientists on numerical methods. Easy step-by-step introduction to numerical solutions. Installation guide for the free software GNU Octave. More than 30 programs (written in Octave) will provide templates for biochemical applications – available as supplementary electronic material. Analysis of experimental data by multi-parameter fits. Correlation of parameters, significance of the results. INDICE: Preparing the computer. Binding equilibria. Binding kinetics. Least squares fit to experimental data. Multi-parameter fits. Comparing the two different approaches.

• ISBN: 978-3-642-20819-5
• Editorial: Springer Berlin Heidelberg
• Encuadernacion: Rústica
• Páginas: 154
• Fecha Publicación: 01/08/2011
• Nº Volúmenes: 1
• Idioma: Inglés