The complex nature of proteins often requires a multitude of sample preparation options. In 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2, Dr. Anton Posch presents a broad coverage of the principles and recent developments of sample preparation and fractionation tools in Expression Proteomics andáspecifically for two-dimensional electrophoresis (2-DE). Detailed laboratorymethods describe the process of sample fractionationáused toáreduce the complexity of the protein sample and the methods of 2-DE analysis and a broad variety of gel-free mass spectrometry-based technologies to detect previously undetectable proteins. Vital informationáregarding protein isolation and preparation for reproducible proteome analysis previously overlooked by scientists has been clearly laid out in this pioneering two-volume set. Volume 1 focuses on the basics of sample preparation and protein labeling techniques with anáemphasis on fractionationáby chemical reagents, chromatography, and electrophoresisámethods. The inclusion of meticulous enrichment strategiesáfurther aids the protein researcher. Invaluable for students of Biochemistry, Biomedicine, Biology and Genomicsand essential for the practicing scientists, 2D PAGE: Sample Preparation and Fractionation, Volumes 1 & 2 delve into the study of proteins with comprehensive and in depth methods and protocols to provide a greater understanding of this intricate and complicated field.
- ISBN: 978-1-61737-742-6
- Editorial: Springer
- Encuadernacion: Rústica
- Páginas: 459
- Fecha Publicación: 01/01/2008
- Nº Volúmenes: 1
- Idioma: Inglés