In the early years of microarray technology, efforts were directed mainly at profiling expressed genes, while recently the microarray platform has been adapted into diverse applications directed toward the investigation of the physical genome. In Microarray Analysis of the Physical Genome: Methods and Protocols, experts in the field cover DNA microarray applications for the detection and characterization of genomic DNA-associated copy number alteration, loss of heterozygosity (LOH), cytosine methylation, protein binding sites, regulatory elements, and replication timing, with an emphasis on higher eukaryote (animal)and cancer genomes. Written in the highly successful Methods in Molecular Biology™ series format, chapters include a brief introduction to their respectivesubjects, lists of the necessary materials and reagents, step-by-step protocols, and time-saving notes on troubleshooting and avoiding known pitfalls. Provides an easily accessible reference volume for microarray-based analyses of the physical genome, written by leading researchers in the field Covers a wide range of microarray platforms and applications Details microarray approaches, ‘wet-bench’ methods, and computational analyses Provides easy to follow microarray protocols, with tips and expert commentary INDICE: From the contents Introduction.- Comparative Genomic Hybridizationon BAC Arrays.- Comparative Genomic Hybridization on Spotted Oligonucleotide Microarrays.- Comparative Genomic Hybridization by Representational Oligonucleotide Microarray Analysis.- Application of Oligonucleotides Arrays for Coincident Comparative Genomic Hybridization, Ploidy Status and Loss of Heterozygosity Studies in Human Cancers.- Molecular Inversion Probe Assay for Allelic Quantitation.- A Whole Genome Amplification Protocol for a Wide Variety of DNAs, Including those from Formalin-Fixed and Paraffin-Embedded Tissue.- Algorithms for Calling Gains and Losses in Array CGH Data.- Methylation Analysis by Microarray.
- ISBN: 978-1-60327-191-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 215
- Fecha Publicación: 01/07/2009
- Nº Volúmenes: 1
- Idioma: Inglés