Proteomic analyses have proven to be an invaluable tool ináobtaining high-throughput protein identification from low-abundance, complex biological samples.In Organelle Proteomics, detailed protocols provideástep-by-step instructionstoásuccessfully study organelle proteomes by performing the purification of the various organelles present in eukaryotic cells, as well asáby preparing certain sub-fractions of organelles. A series of chapters cover the whole analytical procedure of organelle characterization, from its purification starting with whole cells up to protein identification using mass spectrometry. Devoted to methods enabling a global estimate of the reliability of the protein list assigned to an organelle, Organelle Proteomicsáallowsáscientists to gain a vitaland importantáunderstanding of organelle study.
- ISBN: 978-1-61737-772-3
- Editorial: Springer
- Encuadernacion: Rústica
- Páginas: 422
- Fecha Publicación: 01/01/2008
- Nº Volúmenes: 1
- Idioma: Inglés