During recent years, research has greatly expanded our understanding of the sophisticated molecular network of responses which enable plants to develop, survive and propagate under a wide range of conditions. In Plant Signal Transduction: Methods and Protocols, an international panel of experts provide well-established methods vital to analyzing plant signal transduction on the molecular level. Featuring experimental procedures on several of the most popular model organisms, the volume focuses on in planta analyses and the proteins involved in signal transduction in order to aid with the establishment of laboratory techniques or the modification of the protocols for other plants. As part of the highly successful Methods in Molecular Biology™ series, the chapters include brief introductions to the subject, lists of necessary materials, readily reproducible laboratory protocols, and tips on trouble-shooting and avoiding known pitfalls. Offers step-by-step, established and reproducible laboratory protocols written by experts in the field of plant signal transduction and molecular physiology Focuses on novel and emerging techniques to analyze signaling mutants, post-translational protein modification, protein-protein and protein-nucleic acid interactions INDICE: From the contents Multiple Dimensions in Plant Signal Transduction: An Overview.- Phenotyping of Arabidopsis Mutants for Developmental Effects of Gene Deletions.- Phenotyping of Abiotic Responses and Hormone Treatments in Arabidopsis.- Accurate Real-time Reverse Transcription Quantitative PCR.- Probing Spatio-temporal Intracellular Calcium Variations in Plants.- Dynamic RedoxMeasurements with Redox-Sensitive GFP in Plants by Confocal Laser Scanning Microscopy.- Imaging of Reactive Oxygen Species in vivo.- Identification of Thioredoxin Targeted Proteins Using Thioredoxin Single Cysteine Mutant-immobilizedResin.- Determination of in vivo Protein Phosphorylation in Photosynthetic Membranes.- Examining Protein Stability and Its Relevance for Plant Growth and Development.- Probing Circadian Rhythms in Chlamydomonas rheinhardtii by Functional Proteomics.- Bimolecular Fluorescence Complementation (BiFC) to Study Protein-protein Interactions in Living Plant Cells.
- ISBN: 978-1-58829-943-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 320
- Fecha Publicación: 01/10/2008
- Nº Volúmenes: 1
- Idioma: Inglés