Mitochondrial bioenergetics: methods and protocols

Increasing interest in mitochondrial bioenergetics is being driven by the impact of drug and environmental chemical-induced disturbances of mitochondrial function as well as hereditary deficiencies and the progressive deterioration of bioenergetic performance with age. These initiatives have fostered the investigation of genetic and environmental influences on bioenergetics. In Mitochondrial Bioenergetics: Methods and Protocols, researchers in the field detail the practical principles and assays designed to derive quantitative assessment of each set of parameters that reflect different aspects of mitochondrial bioenergetics. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, lists ofthe necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Mitochondrial Bioenergetics: Methods and Protocols helps elevate the quality and rate of investigative discoveries regarding disease states associated with environmental or genetic influences on mitochondrial bioenergetics. Elevates the quality and rate of investigative discoveries regarding disease state associated with enviromental or genetic influences on mitochondrial bioenergentics. Provides step-by-step detail essentail for reproducible results. Contains key notes and implementaion advice from the experts. INDICE: Overview of Mitochondrial Bioenergetics.- Evaluation of Respiration with Clark Type Electrode in Isolated Mitochondria and Permeabilized Animal Cells.- High-Resolution Respirometry. OXPHOS Protocols for Human Cells and Permeabilized Fibres from Small Biopsies of Human Muscle. High Throughput Analysis of Mitochondrial Oxygen Consumption. – Modulation of Cellular Respiration by Endogenously Produced Nitric Oxide in Rat Hippocampal Slices. - Mitochondrial Membrane Potential (DY) Fluctuations Associated with the Metabolic States of Mitochondria. - Safranine as a Fluorescent Probe for the Evaluation of Mitochondrial Membrane Potential in Isolated Organelles and Permeabilized Cells. Fluorescence Measurement of Mitochondrial Membrane Potential Changes in CulturedCells.-Phenomenological Kinetic and Control Analysis of Oxidative Phosphorylation in Isolated Mitochondria.-Expression of Uncoupling Proteins in a Mammalian Cell Culture System (HEK293) and Assessment of their Protein Function.-Measurement of Proton Leak and Electron Leak in Isolated Mitochondria.-Relation between Mitochondrial Membrane Potential and ROS Formation. Use of a Calcium-Sensitive Electrode for Studies on Mitochondrial Calcium Transport. -Imaging Mitochondrial Calcium Signalling with Fluorescent Probes and Single or Two Photon Confocal Microscopy. Mitochondrial Permeability Transition Pore and Calcium Handling.- Imaging of Mitochondrial pH Using SNARF-1. Redox Equivalents and Mitochondrial Bioenergetics.-NMR Methodologies for Studying Mitochondrial Bioenergetics.-Computational Modeling of Mitochondrial Function.

• ISBN: 978-1-61779-381-3
• Editorial: Humana Press
• Encuadernacion: Cartoné
• Páginas: 284
• Fecha Publicación: 28/12/2011
• Nº Volúmenes: 1
• Idioma: Inglés