Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond theresearch of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides.Presents a detailed compendium of techniques used routinely in the leading laboratories utilizing current, common technologies, especially those established for proteomics research Reviews the current practices used for the analysis of the specific single O-GlcNAc found on nuclear and cytoplasmic proteins Disperses the misconception that only experts can analyze the glycome INDICE: From the contents Analysis of N- and O-Linked Glycans from Glycoproteins Using Maldi-TOF Mass Spectrometry.- Infrared Multiphoton Dissociation (IRMPD) Mass Spectrometry for Structural Elucidation of Oligosaccharides.- MassSpectrometry of N-linked Glycans.- Solid-phase Permethylation for Glycomic Analysis.- Method for Investigation of Oligosaccharides Using Phenylhydrazine Derivatization.- 2-Dimensional HPLC Separation with Reverse Phase-Nano-LC-MS/MS for the Characterization of Glycan Pools after Labeling with 2-Aminobenzamide.- Capillary Lectin-Affinity Electrophoresis for Glycan Analysis.- Analysis of Methylated O-Glycan Alditols by CAD-ESI Mass Spectrometry.- High-Throughput and High-Sensitivity Nano-LC/MS and MS/MS for O-Glycan Profiling.- Collision Induced Dissociation Tandem Mass Spectrometry for Structural Elucidation of Glycans.- The Structural Elucidation of Glycosaminoglycans.- Labelling Heparan Sulphate Saccharides with Chromophore, Fluorescence and Mass Tags for HPLC and MS Separations.
- ISBN: 978-1-58829-774-7
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 310
- Fecha Publicación: 01/03/2009
- Nº Volúmenes: 1
- Idioma: Inglés