This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This is the first of three volumes on hydrogen peroxide and cell signaling, and includes chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers hydrogen peroxide and cell signalingContains chapters on such topics as photooxidation of amplex red to resorufin, boronate-based fluorescent probes, and visualization of intracellular hydrogen peroxide with HyPer INDICE: Photooxidation of Amplex Red to Resorufin: Implications of Exposing the Amplex Red Assay to LightFiona A. Summers, Baozhong Zhao, Douglas Ganini, and Ronald P. MasonBoronate-Based Fluorescent Probes: Imaging Hydrogen Peroxide in Living SystemsVivian S. Lin, Bryan C. Dickinson and Christopher J. ChangVisualization of Intracellular Hydrogen Peroxide with HyPer, a Genetically Encoded Fluorescent ProbeIn vivo Imaging of H2O2 Production in DrosophilaSingle Fluorescent Probe Distinguishes Hydrogen Peroxide and Nitric Oxide in Cell ImagingElectrochemical Biosensors for On-Chip Detection of Oxidative StressElectrochemical Detection of H2O2 Formation in Isolated MitochondriaDetection of H2O2 by Fluorescence Correlation SpectroscopyReal-Time Monitoring of Reactive Oxygen and Nitrogen Species in a multi-well plate using the diagnostic marker products of specific probesH2O2 Delivery to Cells: Steady-State versus Bolus AdditionImaging H2O2 Microdomains in Receptor Tyrosine Kinases SignalingThe Determination aAnd Analysis oOf Site-Specific Rates oOf Mitochondrial Reactive Oxygen Species ProductionA Microfluidic Systems Biology Approach for Live Single-Cell Mitochondrial ROS Imaging Detection of Oxidative Damage in Response to Protein Misfolding in the Endoplasmic Reticulum.
- ISBN: 978-0-12-405883-5
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 328
- Fecha Publicación: 19/08/2013
- Nº Volúmenes: 1
- Idioma: Inglés