Due to their high specificity and low toxicity profile, peptides have once again become central to the development of new drugs. In Peptide-Based Drug Design: Methods and Protocols, expert researchers provide a handbook which offers a selection of research and production tools suitable for transforming a promising protein fragment or stand-alone native peptide into a pharmaceutically acceptable composition. The volume delves into contemporary, cutting-edge subjects such as hit isolation and target validation, computer-aided design, sequence modifications to satisfy pharmacologists, in vivo stability and imaging, andthe actual production of difficult sequences. Written in the highly successful Methods in Molecular Biology™ series format, chapters include readily reproducible, step-by-step laboratory protocols, lists of materials, and the Notes section, which highlights tips on troubleshooting and avoiding known pitfalls. Presents full coverage of peptide-based drug development arranged in a sequential order as the leads proceed through the preclinical pipeline Describes innovative and unusual technologies Special focus on peptide drugs in infectious diseases Includes contributions from international leaders in their respective fields Provides a balance of scientific rigor, potential reproducibility and drug development economics INDICE: From the contents Peptide-based Drug Design: Here and Now.- Strategies for the Discovery, Isolation, and Characterization of Natural Bioactive Peptides from the Immune System of Invertebrates.- Sequence Analysis of Antimicrobial Peptides by Tandem Mass Spectrometry.- The Spot Technique: Synthesis and Screening of Peptide Macroarrays on Cellulose Membranes.- Analysis of Abeta interactions using ProteinChip Technology.- NMR in Peptide Drug Development.- Molecular Dynamics Simulations of Peptides.- Short Linear Cationic Antimicrobial Peptides – Screening, Optimizing and Prediction.- Investigation of the Modeof Action of Proline-rich Antimicrobial Peptides: Genetic and Cytometric Approaches.- Serum Stability of Peptides.- Preparation of Glycosylated Amino AcidsSuitable for Fmoc Solid-Phase Assembly.- Synthesis of O-Phosphopeptides on Solid Phase.- Peptidomimetics: Fmoc Solid-Phase Pseudopeptide Synthesis.- Synthesis of Toll-like Receptor-2 Targeting Lipopeptides as Self-Adjuvanting Vaccines.
- ISBN: 978-1-58829-990-1
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 330
- Fecha Publicación: 01/10/2008
- Nº Volúmenes: 1
- Idioma: Inglés