Systems biology is the study of interactions between assorted components of biological systems with the aim of acquiring new insights into how organisms function and respond to different stimuli. Although more and more efforts are being directed toward examining systems biology in complex multi-cellular organisms, the bulk of system-level analyses conducted to date have focused on thebiology of microbes. In, Microbial Systems Biology: Methods and Protocols expert researchers in the field describe the utility and attributes of differenttools (both experimental and computational) that are used for studying microbial systems. Written in the highly successful Methods in Molecular Biology™ series format, chapters include introductions to their respective topics, listsof the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.Authoritative and practical, Microbial Systems Biology: Methods and Protocolsintroduces and aids scientists in using the various tools that are currently available for analysis, modification and utilization of microbial organisms. INDICE: Part I: Sample Prepartion and Identification 1. Flow Cytometry inEnvironmental Microbiology: A Rapid Approach for the Isolation of Single-Cells for Advanced Molecular Biology Analysis Ferrari BC., Winsley T., Bergquist PL. and van Dorst, J. 2. Pressure Cycling Technology in Systems Biology Bradford S. Powell, Alexander V. Lazarev, Greta Carlson, Alexander R. Ivanov, and David A. Rozak 3. Targeted Isolation of Proteins from Natural Microbial Communities Living in an Extreme Environment Steven W. Singer 4. Bacterial Identification and Subtyping Using DNA Microarray and DNA Sequencing Sufian F. Al-Khaldi, Magdi M. Mossoba, Marc M. Allard, E. Kurt Lienau, and Eric D. Brown Part II: Experimental Genomic Analyses 5. Genetic Manipulation of the Obligate Chemolithoautotrophic Bacterium Thiobacillus denitrificans Harry R. Beller, Tina C. Legler, and Staci R. Kane 6. Genome-wide Mapping of the Binding Sites of Proteins that Interact with DNA Stephen Spiro Part III: Protein and Lipid Analyses 7. MICROBIAL PROTEOMICS USING MASS SPECTROMETRY Harry B. Hines 8. Fourier Transform Infrared Spectroscopy for Molecular Analysis of Microbial Cells Jesús J. Ojeda Part IV: Metabolomic Analyses 9. Mass Spectrometry-based Microbial Metabolomics Edward E. K. Baidoo, Peter I. Benke and Jay D. Keasling 10.Fast Sampling of the Cellular Metabolome Walter M. van Gulik, Andre B. Canelas, Hilal Taymaz-Nikerel, Rutger D. Douma, Lodewijk P. de Jonge and Joseph J. Heijnen Part V: Analyses of Cellular Metabolism 11. Metabolic Pathway Determination and Flux Analysis in Non-model Microorganisms through 13C-isotope Labeling Xueyang Feng, Wei-Qin Zhuang, Peter Colletti, Yinjie J. Tang 12. Biolog Phenotype Microarrays April Shea, Mark Wolcott, Simon Daefler, and David Rozak 13. NanoSIP: NanoSIMS Applications for Microbial Biology Jennifer Pett-Ridgeand Peter K. Weber Part VI: Kinetic Modeling of Cellular Processes 14. Electrophysiological-metabolic Modeling of Microbes: Applications in Fuel Cells and Environment Analysis Max Fontus and Peter Ortoleva 15. Simulating Microbial Systems: Addressing Model Uncertainty/Incompleteness via Multiscale and Entropy Methods A. Singharoy, H. Joshi, S. Cheluvaraja, Y. Miao, D. Brown and P. Ortoleva Part VII: Genome-Scale Computational Studies 16. Bacterial Genome Annotation Nicholas Beckloff, Shawn Starkenburg, Tracey Freitas, and Patrick Chain 17. LeishCyc: a Guide to Building a Metabolic Pathway Database and the Visualization of Metabolic Data Eleanor C. Saunders, James I. MacRae, Thomas Naderer, Milica Ng, Malcolm J. McConville and Vladimir A. Liki? 18. Development of Constraint-base System-level Models of Microbial Metabolism Ali Navid 19. Complex Network Analysis in Microbial Systems: Theory and Examples Zahra Zavareh, Eivind Almaas 20. Modeling a Minimal Cell Michael L. Shuler, Patricia Foley, Jordan Atlas
- ISBN: 978-1-61779-826-9
- Editorial: Humana
- Encuadernacion: Cartoné
- Fecha Publicación: 31/05/2012
- Nº Volúmenes: 1
- Idioma: Inglés