Volume Electron Microscopy (vEM), Volume 177 is a collective term for a set of three-dimensional high-resolution ultrastructural imaging techniques that have delivered new insights into biological systems in recent years, garnering substantial interest in the life and clinical sciences. In this book, users will find a variety of vEM workflows and technologies, highlighting application areas with biologically relevant examples. Topics covered include Automated large volume sample preparation for vEM, Resin comparison for Serial Block Face Scanning Volume Electron Microscopy, Immunolabelling for SBF-SEM, Electron Microscopy in Plants, Serial section electron tomography, Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathology, Array tomography, and much more. Other sections focus on Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron Microscopy, Serial Block-face Scanning Electron Microscopy of Schmidtea mediterranea, Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissue, Methods of enhanced FIB-SEM sample preparation and image acquisition, Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopy, and much more. Includes chapters written by key leaders and developers in the field Provides detailed protocols, allowing for the application of workflows in one's own laboratory setting Presents real tips and tricks you won't get from standard research papers INDICE: 1. Automated large volume sample preparation for vEMClaudia S. Lopez2. Resin comparison for Serial Block Face Scanning Volume Electron MicroscopyAnneke Kremer, Saskia Lippens and Chris Geurin3. Immunolabelling for SBF-SEMAlice Liang4. Volume Electron Microscopy in PlantsKirk James Czymmek5. Serial section electron tomographyAndrea Raimondi6. Automated Tape Collecting Ultramicrotomy (ATUM) for targeting neuropathologyMartina Schifferer7. Array tomographyJemima J. Burden and Ian White8. Mitochondria morphometry in 3d datasets of mouse brain obtained with serial block-face Scanning Electron MicroscopyChristel Genoud, Jean-Yves Chatton and Wei Jiao9. Serial Block-face Scanning Electron Microscopy of Schmidtea mediterraneaStephanie Nowotarski10. Correlative multiscale microCT-SBF-SEM imaging of resin-embedded tissueRohan M. Lewis, Orestis L. Katsamenis and Pascale Chavatte-Palmer11. Methods of enhanced FIB-SEM sample preparation and image acquisitionSong Pang12. Functional characterization of endo-lysosomal compartments by correlative live-cell and volume electron microscopyNalan Liv13. Cryo FIB-SEM imagingMichele C. Darrow and James Naismith14. Toward scalable reuse of vEM data: OME-Zarr to the rescueNorman Rzepka, Joshua Allen Moore and John Bogovic15. Public archiving of vEM dataGerard J. Kleywegt and Ardan Patwardhan
- ISBN: 978-0-323-91607-3
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 418
- Fecha Publicación: 23/06/2023
- Nº Volúmenes: 1
- Idioma: Inglés