Many unexplored complex cellular and organismal adaptations occur in responseto the stress of alcohol exposure, and its contribution to the development ofchronic diseases, such as osteoporosis, heart disease and diabetes, is particularly relevant today, given the increased incidence of these diseases in our aging population. In Alcohol: Methods and Protocols, the pleiotropic effects of ethanol in animal and cell culture models are rigorously examined through a collection of detailed procedures written by experts in the field. Sections present clearly defined models of ethanol exposure, recent advances in the development of specific methodologies to mimic the impact of ethanol metabolism in cultured cells, and methodologies to investigate a variety of cells and tissues that are known to be disrupted by ethanol, amongst other topics. Step-by-step protocols for the most commonly used models of acute and chronic ethanol exposure Detailed procedures for cell culture experiments with ethanol Comprehensive methods for studies of fetal alcohol syndrome (FAS) including Zebrafish, avian embryo and rodent models Up-to-date proteomics and micro-array protocols INDICE: From the contents Acute models of ethanol exposure to mice.- A voluntary oral-feeding rat model for pathological alcohol liver injury.- Intragastric ethanol infusion model in rodents.- A practical method of chronic ethanoladministration in mice.- Analysis of ethanol developmental toxicity in Zebrafish.- The avian embryo in fetal alcohol research.- Artificial rearing.- Intragastric intubation of alcohol during the perinatal period.- Human monocytes, macrophages and dendritic cells – alcohol treatment methods.- Generation and useof primary rat cultures for studies of the effects of ethanol.- Development and properties of HepG2 cells which constitutively express CYP2E1.- Modeling the impact of alcohol on cortical development in a dish: Strategies from mappingneural stem cell fate.- Acetaldehyde-induced barrier disruption and paracellular permeability in Caco-2 cell monolayer.- Alcohol-induced oxidative stress in the liver: in vivo measurements.
- ISBN: 978-1-58829-906-2
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 450
- Fecha Publicación: 01/06/2008
- Nº Volúmenes: 1
- Idioma: Inglés