Gene expression can mean the difference between a functional and non-functional genome, between health and disease, and with the development of transgenic crops, the difference between survival and starvation. In DNA-Protein Interactions: Principles and Protocols, Third Edition, this vital subject is brought up to date with protocols exploring the most cutting-edge developments in the field, including in vivo and genome-wide interaction techniques. Addressing topics such as chromatin immunoprecipitation, topological studies, photocrosslinking, FRET and imaging techniques, the volume fully updates and expands upon the successful previous editions. Written in the convenient and informative Methods in Molecular Biology™ series format, chapters include introductions to their respective subjects, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshootingand avoiding known pitfalls. Covers cutting edge techniques in chromatin studies, such as Nucleosome Mapping, Chromatin Immunoprecipitation and ChIP-on-chip Contains many protocols for large scale analysis of genome-protein interaction and long-distance interaction between chromatin domains Describes the use of many physical techniques like Atomic Force Microscopy, FRET, Surface PlasmonResonance and Circular Dichroism INDICE: From the contents Filter-Binding Assays.- Electrophoretic MobilityShift Assays for the Analysis of DNA-Protein Interactions.- DNase I Footprinting.- Exonuclease III Footprinting on Immobilized DNA Templates.- Hydroxyl Radical Footprinting of Protein-DNA Complexes.- The Use of Diethyl Pyrocarbonate (DEPC) and Potassium Permanganate as Probes for Strand Separation and Structural Distortions in DNA.- Uranyl Photofootprinting.- Identification of Protein/DNA Contacts with Dimethyl Sulfate: Methylation Protection and Methylation Interference.- Ethylation Interference Footprinting of DNA-Protein Complexes.- Site-Directed Cleavage of DNA by Protein-Fe(II) EDTA Conjugates within Model Chromatin Complexes.- Identification of Nucleic Acid High Affinity Binding Sequences of Proteins by SELEX.- Identification of Sequence-Specific DNA-Binding Proteins by Southwestern Blotting.- Footprinting DNA-Protein Interactions in Native Polyacrylamide Gels by Chemical Nucleolytic Activity of 1,10-Phenanthroline-Copper.
- ISBN: 978-1-60327-014-4
- Editorial: Humana
- Encuadernacion: Cartoné
- Páginas: 635
- Fecha Publicación: 01/04/2009
- Nº Volúmenes: 1
- Idioma: Inglés