Aptamers can be considered as a valid alternative to antibodies or other bio-mimetic receptors, for the development of biosensors and other analytical methods. The production of aptamers is commonly performed by the SELEX (SystematicEvolution of Ligands by Exponential Enrichment) process, which, starting fromlarge libraries of oligonucleotides, allows the isolation of large amounts offunctional nucleic acids by an iterative process of in vitro selection and subsequent amplification through polymerase chain reaction. Aptamers are suitable for applications based on molecular recognition as analytical, diagnostic and therapeutic tools. The book will focus on the analytical methods which have been developed using aptamers. It will start with an introduction to aptamers and discuss aptamer targets and their general uses. It then goes on to discussdifferent applications , with particular attention to the comparison between aptamer-based biosensors and methods versus the corresponding immunosensors. Examples of aptamer-based diagnostic techniques include whole-cell protein profiling (proteomics), and medical diagnostics for the distinction of diseased versus healthy states. Aptamers can be considered as a valid alternative to antibodies or other bio-mimetic receptors, for the development of biosensors and other analytical methods. The production of aptamers is commonly performed by the SELEX (Systematic Evolution of Ligands by Exponential Enrichment) process, which, starting from large libraries of oligonucleotides, allows the isolationof large amounts of functional nucleic acids by an iterative process of in vitro selection and subsequent amplification through polymerase chain reaction. Aptamers are suitable for applications based on molecular recognition as analytical, diagnostic and therapeutic tools. The book will focus on the analyticalmethods which have been developed using aptamers. Introductive chapters on aptamers will be present. Different applications will be presented with particular attention to the comparison between aptamer-based methods and the corresponding immunosensors.
- ISBN: 978-0-470-14830-3
- Editorial: John Wiley & Sons
- Encuadernacion: Cartoné
- Páginas: 313
- Fecha Publicación: 03/04/2009
- Nº Volúmenes: 1
- Idioma: Inglés