Significant DNA sequence data has been made availabe by several important genome projects. We clearly live in an exciting era, in which the allocation of functions to a mere string of the four letters in the code of life plays a major if not the predominant role of ongoing and future research. In functional genomics techniques are required that allow to examine the occurrence of gene products (be it RNA or protein material) and their interplay with other gene products, if possible, in real time. Again, once an important function has been identified there will be demands to trace or screen that function in in vitro and in vivo systems. For example, it may be necessary to detect dissimilaritiesin RNA expression between individuals or it may be required to detect pathogenes as rapid as possible. Shows a diverse set of instructive examples from probe design to applications in clinical settings guided by experts in the field who provide easy to follow experiments The book commences with an introductionto the basic principles of fluorescence Extends the potential of realtime PCRContributions advocate simplifications of the probe technologies INDICE: From the contents Basic Principles of Fluorescence and Energy Transfer.- Multiplex Detection of Mutations.- Detection of Salmonella species in foodstuffs.- Identification of homozygous transgenic mice by genomic real time PCR.- Quantitative analysis of gene expression relative to 18S rRNA in carcinoma samples using the LightCyclerÒ instrument and a SYBR GreenI based assay: determining FAT10 mRNA levels in hepatocellular carcinoma.- Absolute quantitation of Feline Leukemia Virus (FeLV) proviral DNA and viral RNA loads by TaqMan® Real-time PCR and RT-PCR.- High throughput real-time PCR.-
- ISBN: 978-1-58829-700-6
- Editorial: HUMANA PRESS
- Encuadernacion: Cartoné
- Páginas: 274
- Fecha Publicación: 01/01/2008
- Nº Volúmenes: 1
- Idioma: Inglés