This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers cilia and includes chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium. Continues the legacy of this premier serial with quality chapters authored by leaders in the field Covers ciliaContains chapters on such topics as electron microscopy of IFT in cilia and flagella, radial spoke isolation and assays, and biomechanical measurements of kinocilium INDICE: Electron microscopy of IFT in cilia and flagellaBiochemical analysis of ciliary transport Visualization of IFT in Chlamydomonas flagella Visualization of kidney cilia-mediated calcium signaling Methods for analyzing nodal cilia and flow Methods for building synthetic cilia-like motor-microtubule arrays Isolation of IFT particle proteins from Chlamydomonas Radial Spoke isolation and assays Biomechanical measurements of kinocilium Methods for analysis of Calcium/calmodulin signaling in flagella Ubiquitination of Flagellar Proteins Visualizing IFT in C. elegans cilia Proteomic analysis of mammalian primary cilia TSC and TOR signaling in cilia Bioinformatic approaches to cilia Methods for testing cilia function in cell migration Signaling and waveform in Drosophila sperm flagella Analysis of import into primary cilia Patch-Clamp Recording from Mouse and Human Spermatozoa Optical trap measurements of flagellar membrane motility
- ISBN: 978-0-12-397945-2
- Editorial: Academic Press
- Encuadernacion: Cartoné
- Páginas: 472
- Fecha Publicación: 08/05/2013
- Nº Volúmenes: 1
- Idioma: Inglés