Methods for Monitoring Mitochondrial Functions During Pathogen Infection

Methods for Monitoring Mitochondrial Functions During Pathogen Infection, Volume 188 in the Methods in Cell Biology series highlights advances in the field, with this new volume presenting interesting chapters on timely topics, including Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial Transfer, Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophila, Quantitative assessment of mitochondrial membrane potential in macrophages in sepsis, Quantification of mitochondrial reactive oxygen species in macrophages during sepsis, and much more.Additional chapters cover Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory response, Analysis of cytosolic mtDNA release during Staphylococcus Aureus infection, Monitoring mitochondria dynamics upon infection using a tomographic approach, A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cells, Isolation of mitochondria from Mycobacteria infected cells, Monitoring mitochondrial localization of Mycobacterial proteins, and more. Provides the authority and expertise of leading contributors from an international board of authorsPresents the latest release in the Methods in Cell Biology seriesIncludes the latest information on the topic of development, characterization, and applications in CAR T Cells INDICE: 1. Mitochondrial Labeling with Fluorescent Proteins for Enhanced Morphological Characterization and Visualization of Intercellular Mitochondrial TransferToshio Miki2. Visualizing mitochondrial electron transport chain complexes and super-complexes during infection of human macrophages with Legionella pneumophilaPedro Escoll3. Quantitative assessment of mitochondrial membrane potential in macrophages in sepsisGuochang Hu4. Quantification of mitochondrial reactive oxygen species in macrophages during sepsisGuochang Hu5. Quantification of intracellular and mitochondrial ATP content in macrophages during lipopolysaccharide-induced inflammatory responseGuochang Hu6. Analysis of cytosolic mtDNA release during Staphylococcus Aureus infectionSaverio Marchi7. Monitoring mitochondria dynamics upon infection using a tomographic approachGianluca Morroni8. A screening system to determine bacterial effects on MADCAM1 expression by high endothelial venule cellsOliver Kepp9. Isolation of mitochondria from Mycobacteria infected cellsKrishnaveni Mohareer10. Monitoring mitochondrial localization of Mycobacterial proteinsKrishnaveni Mohareer11. Bacterial predators and BALOs: growth protocol and relation with mithochondriaValerio Iebba12. Assessment of adhering and invading properties of Escherichia coli strainsValerio Iebba13. Bioinformatic techniques to study intracellular bacteriaValerio Iebba

• ISBN: 978-0-323-99219-0
• Editorial: Academic Press
• Encuadernacion: Cartoné
• Fecha Publicación: 01/12/2024
• Nº Volúmenes: 1
• Idioma: Inglés